Imbalance of proresolving lipid mediators in persistent allodynia dissociated from signs of clinical arthritis
Copyright © 2020 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the International Association for the Study of Pain..
ABSTRACT: Rheumatoid arthritis-associated pain is poorly managed, often persisting when joint inflammation is pharmacologically controlled. Comparably, in the mouse K/BxN serum-transfer model of inflammatory arthritis, hind paw nociceptive hypersensitivity occurs with ankle joint swelling (5 days after immunisation) persisting after swelling has resolved (25 days after immunisation). In this study, lipid mediator (LM) profiling of lumbar dorsal root ganglia (DRG), the site of sensory neuron cell bodies innervating the ankle joints, 5 days and 25 days after serum transfer demonstrated a shift in specialised proresolving LM profiles. Persistent nociception without joint swelling was associated with low concentrations of the specialised proresolving LM Maresin 1 (MaR1) and high macrophage numbers in DRG. MaR1 application to cultured DRG neurons inhibited both capsaicin-induced increase of intracellular calcium ions and release of calcitonin gene-related peptide in a dose-dependent manner. Furthermore, in peritoneal macrophages challenged with lipopolysaccharide, MaR1 reduced proinflammatory cytokine expression. Systemic MaR1 administration caused sustained reversal of nociceptive hypersensitivity and reduced inflammatory macrophage numbers in DRG. Unlike gabapentin, which was used as positive control, systemic MaR1 did not display acute antihyperalgesic action. Therefore, these data suggest that MaR1 effects observed after K/BxN serum transfer relate to modulation of macrophage recruitment, more likely than to direct actions on sensory neurons. Our study highlights that, in DRG, aberrant proresolution mechanisms play a key role in arthritis joint pain dissociated from joint swelling, opening novel approaches for rheumatoid arthritis pain treatment.
| Errataetall: | |
|---|---|
| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2020 |
|---|---|
| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:161 |
|---|---|
| Enthalten in: |
Pain - 161(2020), 9 vom: 01. Sept., Seite 2155-2166 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Allen, Benjamin L [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Calcitonin Gene-Related Peptide |
|---|
| Anmerkungen: |
Date Completed 14.05.2021 Date Revised 22.03.2023 published: Print ErratumIn: Pain. 2021 Sep 1;162(9):2456. - PMID 34448758 Citation Status MEDLINE |
|---|
| doi: |
10.1097/j.pain.0000000000001908 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM309625300 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM309625300 | ||
| 003 | DE-627 | ||
| 005 | 20231225134327.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1097/j.pain.0000000000001908 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1032.xml |
| 035 | |a (DE-627)NLM309625300 | ||
| 035 | |a (NLM)32379221 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Allen, Benjamin L |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Imbalance of proresolving lipid mediators in persistent allodynia dissociated from signs of clinical arthritis |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 14.05.2021 | ||
| 500 | |a Date Revised 22.03.2023 | ||
| 500 | |a published: Print | ||
| 500 | |a ErratumIn: Pain. 2021 Sep 1;162(9):2456. - PMID 34448758 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2020 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the International Association for the Study of Pain. | ||
| 520 | |a ABSTRACT: Rheumatoid arthritis-associated pain is poorly managed, often persisting when joint inflammation is pharmacologically controlled. Comparably, in the mouse K/BxN serum-transfer model of inflammatory arthritis, hind paw nociceptive hypersensitivity occurs with ankle joint swelling (5 days after immunisation) persisting after swelling has resolved (25 days after immunisation). In this study, lipid mediator (LM) profiling of lumbar dorsal root ganglia (DRG), the site of sensory neuron cell bodies innervating the ankle joints, 5 days and 25 days after serum transfer demonstrated a shift in specialised proresolving LM profiles. Persistent nociception without joint swelling was associated with low concentrations of the specialised proresolving LM Maresin 1 (MaR1) and high macrophage numbers in DRG. MaR1 application to cultured DRG neurons inhibited both capsaicin-induced increase of intracellular calcium ions and release of calcitonin gene-related peptide in a dose-dependent manner. Furthermore, in peritoneal macrophages challenged with lipopolysaccharide, MaR1 reduced proinflammatory cytokine expression. Systemic MaR1 administration caused sustained reversal of nociceptive hypersensitivity and reduced inflammatory macrophage numbers in DRG. Unlike gabapentin, which was used as positive control, systemic MaR1 did not display acute antihyperalgesic action. Therefore, these data suggest that MaR1 effects observed after K/BxN serum transfer relate to modulation of macrophage recruitment, more likely than to direct actions on sensory neurons. Our study highlights that, in DRG, aberrant proresolution mechanisms play a key role in arthritis joint pain dissociated from joint swelling, opening novel approaches for rheumatoid arthritis pain treatment | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Calcitonin Gene-Related Peptide |2 NLM | |
| 650 | 7 | |a JHB2QIZ69Z |2 NLM | |
| 700 | 1 | |a Montague-Cardoso, Karli |e verfasserin |4 aut | |
| 700 | 1 | |a Simeoli, Raffaele |e verfasserin |4 aut | |
| 700 | 1 | |a Colas, Romain A |e verfasserin |4 aut | |
| 700 | 1 | |a Oggero, Silvia |e verfasserin |4 aut | |
| 700 | 1 | |a Vilar, Bruno |e verfasserin |4 aut | |
| 700 | 1 | |a McNaughton, Peter A |e verfasserin |4 aut | |
| 700 | 1 | |a Dalli, Jesmond |e verfasserin |4 aut | |
| 700 | 1 | |a Perretti, Mauro |e verfasserin |4 aut | |
| 700 | 1 | |a Sher, Emanuele |e verfasserin |4 aut | |
| 700 | 1 | |a Malcangio, Marzia |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Pain |d 1993 |g 161(2020), 9 vom: 01. Sept., Seite 2155-2166 |w (DE-627)NLM000179205 |x 1872-6623 |7 nnns |
| 773 | 1 | 8 | |g volume:161 |g year:2020 |g number:9 |g day:01 |g month:09 |g pages:2155-2166 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1097/j.pain.0000000000001908 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 161 |j 2020 |e 9 |b 01 |c 09 |h 2155-2166 | ||