Derivatives of usnic acid cause cytostatic effect in Caco-2 cells
Usnic acid has anti-cancer activity, however, low solubility and toxicity limit the potential. To investigate biological activity of usnic acid derivatives, enantiopure derivatives were synthesised by reacting usnic acid with ethylenediamine, which yielded one dimer product ((+)-1), and two tetra cyclic compounds ((+)-2 and (-)-2). The products were characterised with NMR, and evaluated in vitro in human colon cancer cell line Caco-2 by cell count, phase-contrast microscopy, MTT-assay, measurement of DNA content and cell cycle distribution. All compounds tested showed cytostatic effect in Caco-2 cells, but each compound had a distinct cellular effect. Compound (+)-1 showed anti-proliferative activity by increasing the percentage of cells in S-phase with 25% compared to the control. Compounds (+)-2 and (-)-2 induced paraptosis, but only compound (+)-2 modulated cell cycle distribution by accumulating cells in G2/M-phase by 47% and reduced DNA content by 60%. All compounds express interesting cellular and potential anti-proliferative activity.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2021 |
---|---|
Erschienen: |
2021 |
Enthalten in: |
Zur Gesamtaufnahme - volume:35 |
---|---|
Enthalten in: |
Natural product research - 35(2021), 23 vom: 01. Dez., Seite 4953-4959 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Samuelsen, Lisa [VerfasserIn] |
---|
Links: |
---|
Themen: |
0W584PFJ77 |
---|
Anmerkungen: |
Date Completed 03.12.2021 Date Revised 14.12.2021 published: Print-Electronic Citation Status MEDLINE |
---|
doi: |
10.1080/14786419.2020.1756796 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM309360943 |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | NLM309360943 | ||
003 | DE-627 | ||
005 | 20231225133736.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231225s2021 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1080/14786419.2020.1756796 |2 doi | |
028 | 5 | 2 | |a pubmed24n1031.xml |
035 | |a (DE-627)NLM309360943 | ||
035 | |a (NLM)32352327 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Samuelsen, Lisa |e verfasserin |4 aut | |
245 | 1 | 0 | |a Derivatives of usnic acid cause cytostatic effect in Caco-2 cells |
264 | 1 | |c 2021 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 03.12.2021 | ||
500 | |a Date Revised 14.12.2021 | ||
500 | |a published: Print-Electronic | ||
500 | |a Citation Status MEDLINE | ||
520 | |a Usnic acid has anti-cancer activity, however, low solubility and toxicity limit the potential. To investigate biological activity of usnic acid derivatives, enantiopure derivatives were synthesised by reacting usnic acid with ethylenediamine, which yielded one dimer product ((+)-1), and two tetra cyclic compounds ((+)-2 and (-)-2). The products were characterised with NMR, and evaluated in vitro in human colon cancer cell line Caco-2 by cell count, phase-contrast microscopy, MTT-assay, measurement of DNA content and cell cycle distribution. All compounds tested showed cytostatic effect in Caco-2 cells, but each compound had a distinct cellular effect. Compound (+)-1 showed anti-proliferative activity by increasing the percentage of cells in S-phase with 25% compared to the control. Compounds (+)-2 and (-)-2 induced paraptosis, but only compound (+)-2 modulated cell cycle distribution by accumulating cells in G2/M-phase by 47% and reduced DNA content by 60%. All compounds express interesting cellular and potential anti-proliferative activity | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Synthesis | |
650 | 4 | |a anti-cancer effect | |
650 | 4 | |a cell cycle distribution | |
650 | 4 | |a cellular response | |
650 | 4 | |a enantiomers | |
650 | 4 | |a paraptosis | |
650 | 7 | |a Benzofurans |2 NLM | |
650 | 7 | |a Cytostatic Agents |2 NLM | |
650 | 7 | |a usnic acid |2 NLM | |
650 | 7 | |a 0W584PFJ77 |2 NLM | |
700 | 1 | |a Hansen, Poul Erik |e verfasserin |4 aut | |
700 | 1 | |a Vang, Ole |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Natural product research |d 2003 |g 35(2021), 23 vom: 01. Dez., Seite 4953-4959 |w (DE-627)NLM124466303 |x 1478-6427 |7 nnns |
773 | 1 | 8 | |g volume:35 |g year:2021 |g number:23 |g day:01 |g month:12 |g pages:4953-4959 |
856 | 4 | 0 | |u http://dx.doi.org/10.1080/14786419.2020.1756796 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 35 |j 2021 |e 23 |b 01 |c 12 |h 4953-4959 |