Optimization of a fluorescent qPCR detection for RNA of SARS-CoV-2
We optimized a fluorescent quantitative polymerase chain reaction (qPCR) assay system for rapid and real time detection of SARS-CoV-2 RNA. The results show that the lowest dilution of RNA samples used for the detection of SARS-CoV-2 RNA could reach 1/10 000 (the initial value is set as 10 ng/μL). Moreover, the cycle threshold (Ct) for samples of clinically diagnosed COVID-19 was lower than 35 or 40. The sensitivity of this method was satisfactory. The results were consistent with those of the COVID-19 detection kit on the market under the same conditions, but the number of cycles required was shortened by about 2. Therefore, the optimized assay developed in this study can be used in screening and early clinical diagnosis. Our work provides a tool to facilitate rapid clinical diagnosis of COVID-19.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:36 |
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| Enthalten in: |
Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 36(2020), 4 vom: 25. Apr., Seite 732-739 |
| Sprache: |
Chinesisch |
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| Beteiligte Personen: |
Li, Xuelong [VerfasserIn] |
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| Links: |
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| Themen: |
COVID-19 |
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| Anmerkungen: |
Date Completed 01.05.2020 Date Revised 18.12.2020 published: Print Citation Status MEDLINE |
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| doi: |
10.13345/j.cjb.200088 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM309309611 |
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| 245 | 1 | 0 | |a Optimization of a fluorescent qPCR detection for RNA of SARS-CoV-2 |
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| 500 | |a Date Revised 18.12.2020 | ||
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a We optimized a fluorescent quantitative polymerase chain reaction (qPCR) assay system for rapid and real time detection of SARS-CoV-2 RNA. The results show that the lowest dilution of RNA samples used for the detection of SARS-CoV-2 RNA could reach 1/10 000 (the initial value is set as 10 ng/μL). Moreover, the cycle threshold (Ct) for samples of clinically diagnosed COVID-19 was lower than 35 or 40. The sensitivity of this method was satisfactory. The results were consistent with those of the COVID-19 detection kit on the market under the same conditions, but the number of cycles required was shortened by about 2. Therefore, the optimized assay developed in this study can be used in screening and early clinical diagnosis. Our work provides a tool to facilitate rapid clinical diagnosis of COVID-19 | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a COVID-19 | |
| 650 | 4 | |a N gene | |
| 650 | 4 | |a ORF1a gene | |
| 650 | 4 | |a SARS-CoV-2 | |
| 650 | 4 | |a fluorescence real-time quantitative PCR | |
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| 700 | 1 | |a Liu, Junhua |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Qianyang |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Lin |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Shanshan |e verfasserin |4 aut | |
| 700 | 1 | |a Yin, Xiushan |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Sheng wu gong cheng xue bao = Chinese journal of biotechnology |d 1997 |g 36(2020), 4 vom: 25. Apr., Seite 732-739 |w (DE-627)NLM093700067 |x 1872-2075 |7 nnns |
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