Campylobacter in chicken - Critical parameters for international, multicentre evaluation of air sampling and detection methods
Copyright © 2020 Elsevier Ltd. All rights reserved..
The present pilot study aimed at evaluating air sampling as a novel method for monitoring Campylobacter in poultry farms. We compared the bacteriological isolation of Campylobacter from boot swabs and air filter samples using ISO 10272-1:2017. A secondary aim was to evaluate the use of molecular methods, i.e. real time PCR, on the same sample set. Samples from 44 flocks from five European countries were collected, and included air samples, in parallel with boot swabs. Campylobacter spp. was isolated from seven of 44 boot swabs from three of five partners using the enrichment method. Two of these positive boot swab samples had corresponding positive air samples. Using enrichment, one positive air sample was negative in the corresponding boot swabs, but Campylobacter spp. was isolated from direct plating of the boot swab sample. One partner isolated Campylobacter spp. from six of 10 boot swabs using direct plating. Overall, 33 air filter samples were screened directly with PCR, returning 14 positive results. In conclusion, there was a lack of correspondence between results from analysis of boot swabs and air filters using ISO 10272-1:2017. In contrast, the combination of air filters and direct real-time PCR might be a way forward. Despite the use of the detailed ISO protocols, there were still sections that could be interpreted differently among laboratories. Air sampling may turn into a multi-purpose and low-cost sampling method that may be integrated into self-monitoring programs.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2020 |
|---|---|
| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:90 |
|---|---|
| Enthalten in: |
Food microbiology - 90(2020) vom: 21. Sept., Seite 103455 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Johannessen, Gro S [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Air sampling |
|---|
| Anmerkungen: |
Date Completed 09.11.2020 Date Revised 09.11.2020 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.fm.2020.103455 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM309204542 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM309204542 | ||
| 003 | DE-627 | ||
| 005 | 20231225133410.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.fm.2020.103455 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1030.xml |
| 035 | |a (DE-627)NLM309204542 | ||
| 035 | |a (NLM)32336358 | ||
| 035 | |a (PII)S0740-0020(20)30044-7 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Johannessen, Gro S |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Campylobacter in chicken - Critical parameters for international, multicentre evaluation of air sampling and detection methods |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 09.11.2020 | ||
| 500 | |a Date Revised 09.11.2020 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2020 Elsevier Ltd. All rights reserved. | ||
| 520 | |a The present pilot study aimed at evaluating air sampling as a novel method for monitoring Campylobacter in poultry farms. We compared the bacteriological isolation of Campylobacter from boot swabs and air filter samples using ISO 10272-1:2017. A secondary aim was to evaluate the use of molecular methods, i.e. real time PCR, on the same sample set. Samples from 44 flocks from five European countries were collected, and included air samples, in parallel with boot swabs. Campylobacter spp. was isolated from seven of 44 boot swabs from three of five partners using the enrichment method. Two of these positive boot swab samples had corresponding positive air samples. Using enrichment, one positive air sample was negative in the corresponding boot swabs, but Campylobacter spp. was isolated from direct plating of the boot swab sample. One partner isolated Campylobacter spp. from six of 10 boot swabs using direct plating. Overall, 33 air filter samples were screened directly with PCR, returning 14 positive results. In conclusion, there was a lack of correspondence between results from analysis of boot swabs and air filters using ISO 10272-1:2017. In contrast, the combination of air filters and direct real-time PCR might be a way forward. Despite the use of the detailed ISO protocols, there were still sections that could be interpreted differently among laboratories. Air sampling may turn into a multi-purpose and low-cost sampling method that may be integrated into self-monitoring programs | ||
| 650 | 4 | |a Evaluation Study | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Multicenter Study | |
| 650 | 4 | |a Air sampling | |
| 650 | 4 | |a Food | |
| 650 | 4 | |a ISO | |
| 650 | 4 | |a PCR | |
| 650 | 4 | |a Standardization | |
| 650 | 4 | |a Zoonoses | |
| 700 | 1 | |a Garofolo, Giuliano |e verfasserin |4 aut | |
| 700 | 1 | |a Di Serafino, Gabriella |e verfasserin |4 aut | |
| 700 | 1 | |a Koláčková, Ivana |e verfasserin |4 aut | |
| 700 | 1 | |a Karpíšková, Renáta |e verfasserin |4 aut | |
| 700 | 1 | |a Wieczorek, Kinga |e verfasserin |4 aut | |
| 700 | 1 | |a Osek, Jacek |e verfasserin |4 aut | |
| 700 | 1 | |a Christensen, Julia |e verfasserin |4 aut | |
| 700 | 1 | |a Torp, Mona |e verfasserin |4 aut | |
| 700 | 1 | |a Hoorfar, Jeffrey |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Food microbiology |d 1996 |g 90(2020) vom: 21. Sept., Seite 103455 |w (DE-627)NLM085753173 |x 1095-9998 |7 nnns |
| 773 | 1 | 8 | |g volume:90 |g year:2020 |g day:21 |g month:09 |g pages:103455 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.fm.2020.103455 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 90 |j 2020 |b 21 |c 09 |h 103455 | ||