A field indoor air measurement of SARS-CoV-2 in the patient rooms of the largest hospital in Iran
Copyright © 2020 Elsevier B.V. All rights reserved..
The coronavirus disease 2019 (COVID-19) emerged in Wuhan city, China, in late 2019 and has rapidly spread throughout the world. The major route of transmission of SARS-CoV-2 is in contention, with the airborne route a likely transmission pathway for carrying the virus within indoor environments. Until now, there has been no evidence for detection of airborne severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and this may have implication for the potential spread of the COVID-19. We investigated the air of patient rooms with confirmed COVID-19 in the largest hospital in Iran, on March 17, 2020. To collect the SARS-CoV-2 particles, ten air samples were collected into the sterile standard midget impingers containing 20 mL DMEM with 100 μg/mL streptomycin, 100 U/mL penicillin and 1% antifoam reagent for 1 h. Besides, indoor particle number concentrations, CO2, relative humidity and temperature were recorded throughout the sampling duration. Viral RNA was extracted from samples taken from the impingers and Reverse-Transcription PCR (RT-PCR) was applied to confirm the positivity of collected samples based on the virus genome sequence. Fortunately, in this study all air samples which were collected 2 to 5 m from the patients' beds with confirmed COVID-19 were negative. Despite we indicated that all air samples were negative, however, we suggest further in vivo experiments should be conducted using actual patient cough, sneeze and breath aerosols in order to show the possibility of generation of the airborne size carrier aerosols and the viability fraction of the embedded virus in those carrier aerosols.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:725 |
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| Enthalten in: |
The Science of the total environment - 725(2020) vom: 10. Juli, Seite 138401 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Faridi, Sasan [VerfasserIn] |
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| Anmerkungen: |
Date Completed 01.06.2020 Date Revised 13.11.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.scitotenv.2020.138401 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM308683382 |
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| 520 | |a The coronavirus disease 2019 (COVID-19) emerged in Wuhan city, China, in late 2019 and has rapidly spread throughout the world. The major route of transmission of SARS-CoV-2 is in contention, with the airborne route a likely transmission pathway for carrying the virus within indoor environments. Until now, there has been no evidence for detection of airborne severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and this may have implication for the potential spread of the COVID-19. We investigated the air of patient rooms with confirmed COVID-19 in the largest hospital in Iran, on March 17, 2020. To collect the SARS-CoV-2 particles, ten air samples were collected into the sterile standard midget impingers containing 20 mL DMEM with 100 μg/mL streptomycin, 100 U/mL penicillin and 1% antifoam reagent for 1 h. Besides, indoor particle number concentrations, CO2, relative humidity and temperature were recorded throughout the sampling duration. Viral RNA was extracted from samples taken from the impingers and Reverse-Transcription PCR (RT-PCR) was applied to confirm the positivity of collected samples based on the virus genome sequence. Fortunately, in this study all air samples which were collected 2 to 5 m from the patients' beds with confirmed COVID-19 were negative. Despite we indicated that all air samples were negative, however, we suggest further in vivo experiments should be conducted using actual patient cough, sneeze and breath aerosols in order to show the possibility of generation of the airborne size carrier aerosols and the viability fraction of the embedded virus in those carrier aerosols | ||
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| 650 | 4 | |a Outbreak | |
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| 650 | 4 | |a Tehran | |
| 700 | 1 | |a Niazi, Sadegh |e verfasserin |4 aut | |
| 700 | 1 | |a Sadeghi, Kaveh |e verfasserin |4 aut | |
| 700 | 1 | |a Naddafi, Kazem |e verfasserin |4 aut | |
| 700 | 1 | |a Yavarian, Jila |e verfasserin |4 aut | |
| 700 | 1 | |a Shamsipour, Mansour |e verfasserin |4 aut | |
| 700 | 1 | |a Jandaghi, Nazanin Zahra Shafiei |e verfasserin |4 aut | |
| 700 | 1 | |a Sadeghniiat, Khosro |e verfasserin |4 aut | |
| 700 | 1 | |a Nabizadeh, Ramin |e verfasserin |4 aut | |
| 700 | 1 | |a Yunesian, Masud |e verfasserin |4 aut | |
| 700 | 1 | |a Momeniha, Fatemeh |e verfasserin |4 aut | |
| 700 | 1 | |a Mokamel, Adel |e verfasserin |4 aut | |
| 700 | 1 | |a Hassanvand, Mohammad Sadegh |e verfasserin |4 aut | |
| 700 | 1 | |a MokhtariAzad, Talat |e verfasserin |4 aut | |
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