Bactrian camel serum albumins-based nanocomposite as versatile biocargo for drug delivery, biocatalysis and detection of hydrogen peroxide
Copyright © 2020 Elsevier B.V. All rights reserved..
In response to extreme environmental conditions, Bactrian camels with largest population in China have evolved with the unique and extraordinary stress-tolerant mechanism in the bodies, in which the most abundantly secreted serum albumins contribute to an important role in diverse physiological activities such as maintaining osmotic pressure and transporting endogenous/exogenous molecules. In this study, we have for the first time purified Chinese Bactrian camel serum albumins (CSA) aimed at exploring their biomedical application. The mass spectrometric as well as structural analysis of CSA have revealed the sequence consensus and alpha-helix abundant structures among its heterologous proteins. Using desolvation methods, CSA-based nanoparticles have been prepared to encapsulate two substrate molecules including Doxorubicin (Dox) and hemin, which confers the versatility of nanocomposite. As drug delivery matrix, the Dox-loaded CSA nanoparticles displayed sustained release behaviors of DOX with the decreased cytotoxicity detected by both CCK-8 assay and real-time cell analysis. The CSA-hemin nanoparticles exhibited superior catalytic activities in the oxidation of Orange II comparable with horse radish peroxidase following a ping-pong mechanism. Furthermore, the constructed CSA-hemin nanoparticles were applied for the spectroscopic detection of H2O2 resulting in a wide linear calibration curve ranging from 5 to 400 μM with a detection limit of 3.32 μM.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:109 |
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| Enthalten in: |
Materials science & engineering. C, Materials for biological applications - 109(2020) vom: 01. Apr., Seite 110627 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Yu, Xinyu [VerfasserIn] |
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| Links: |
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| Themen: |
743LRP9S7N |
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| Anmerkungen: |
Date Completed 16.12.2020 Date Revised 16.12.2020 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.msec.2020.110627 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a In response to extreme environmental conditions, Bactrian camels with largest population in China have evolved with the unique and extraordinary stress-tolerant mechanism in the bodies, in which the most abundantly secreted serum albumins contribute to an important role in diverse physiological activities such as maintaining osmotic pressure and transporting endogenous/exogenous molecules. In this study, we have for the first time purified Chinese Bactrian camel serum albumins (CSA) aimed at exploring their biomedical application. The mass spectrometric as well as structural analysis of CSA have revealed the sequence consensus and alpha-helix abundant structures among its heterologous proteins. Using desolvation methods, CSA-based nanoparticles have been prepared to encapsulate two substrate molecules including Doxorubicin (Dox) and hemin, which confers the versatility of nanocomposite. As drug delivery matrix, the Dox-loaded CSA nanoparticles displayed sustained release behaviors of DOX with the decreased cytotoxicity detected by both CCK-8 assay and real-time cell analysis. The CSA-hemin nanoparticles exhibited superior catalytic activities in the oxidation of Orange II comparable with horse radish peroxidase following a ping-pong mechanism. Furthermore, the constructed CSA-hemin nanoparticles were applied for the spectroscopic detection of H2O2 resulting in a wide linear calibration curve ranging from 5 to 400 μM with a detection limit of 3.32 μM | ||
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| 700 | 1 | |a Chen, Jin |e verfasserin |4 aut | |
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