Overexpression of phzM contributes to much more production of pyocyanin converted from phenazine-1-carboxylic acid in the absence of RpoS in Pseudomonas aeruginosa
Pyocyanin produced by Pseudomonas aeruginosa is a key virulence factor that often causes heavy damages to airway and lung in patients. Conversion of phenazine-1-carboxylic acid to pyocyanin involves an extrametabolic pathway that contains two enzymes encoded, respectively, by phzM and phzS. In this study, with construction of the rpoS-deficient mutant, we first found that although phenazine production increased, pyocyanin produced in the mutant YTΔrpoS was fourfold much higher than that in the wild-type strain YT. To investigate this issue, we constructed phzM-lacZ fusion on a vector and on the chromosome. By quantifying β-galactosidase activities, we confirmed that expression of the phzM was up-regulated when the rpoS gene was inactivated. However, no changes occurred in the expression of phzS and phzH when the rpoS was knocked out. Taken together, overproduction of the SAM-dependent methyltransferase (PhzM) might contribute to the increased pyocyanin in the absence of RpoS in P. aeruginosa.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2020 |
|---|---|
| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:202 |
|---|---|
| Enthalten in: |
Archives of microbiology - 202(2020), 6 vom: 13. Aug., Seite 1507-1515 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Wang, Kewen [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 24.08.2020 Date Revised 21.05.2021 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1007/s00203-020-01837-8 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM308085086 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM308085086 | ||
| 003 | DE-627 | ||
| 005 | 20231225130959.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s00203-020-01837-8 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1026.xml |
| 035 | |a (DE-627)NLM308085086 | ||
| 035 | |a (NLM)32222778 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Wang, Kewen |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Overexpression of phzM contributes to much more production of pyocyanin converted from phenazine-1-carboxylic acid in the absence of RpoS in Pseudomonas aeruginosa |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 24.08.2020 | ||
| 500 | |a Date Revised 21.05.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Pyocyanin produced by Pseudomonas aeruginosa is a key virulence factor that often causes heavy damages to airway and lung in patients. Conversion of phenazine-1-carboxylic acid to pyocyanin involves an extrametabolic pathway that contains two enzymes encoded, respectively, by phzM and phzS. In this study, with construction of the rpoS-deficient mutant, we first found that although phenazine production increased, pyocyanin produced in the mutant YTΔrpoS was fourfold much higher than that in the wild-type strain YT. To investigate this issue, we constructed phzM-lacZ fusion on a vector and on the chromosome. By quantifying β-galactosidase activities, we confirmed that expression of the phzM was up-regulated when the rpoS gene was inactivated. However, no changes occurred in the expression of phzS and phzH when the rpoS was knocked out. Taken together, overproduction of the SAM-dependent methyltransferase (PhzM) might contribute to the increased pyocyanin in the absence of RpoS in P. aeruginosa | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Phenazine-1-carboxylic acid | |
| 650 | 4 | |a Pseudomonas aeruginosa | |
| 650 | 4 | |a Pyocyanin | |
| 650 | 4 | |a RpoS | |
| 650 | 4 | |a phzM | |
| 650 | 7 | |a Bacterial Proteins |2 NLM | |
| 650 | 7 | |a Phenazines |2 NLM | |
| 650 | 7 | |a Sigma Factor |2 NLM | |
| 650 | 7 | |a Virulence Factors |2 NLM | |
| 650 | 7 | |a sigma factor KatF protein, Bacteria |2 NLM | |
| 650 | 7 | |a 1-phenazinecarboxylic acid |2 NLM | |
| 650 | 7 | |a 2538-68-3 |2 NLM | |
| 650 | 7 | |a Pyocyanine |2 NLM | |
| 650 | 7 | |a 9OQM399341 |2 NLM | |
| 650 | 7 | |a Mixed Function Oxygenases |2 NLM | |
| 650 | 7 | |a EC 1.- |2 NLM | |
| 650 | 7 | |a PhzS protein, Pseudomonas aeruginosa |2 NLM | |
| 650 | 7 | |a EC 1.14.13.- |2 NLM | |
| 650 | 7 | |a Methyltransferases |2 NLM | |
| 650 | 7 | |a EC 2.1.1.- |2 NLM | |
| 650 | 7 | |a PhzM protein, Pseudomonas aeruginosa |2 NLM | |
| 650 | 7 | |a EC 2.1.1.- |2 NLM | |
| 700 | 1 | |a Kai, Le |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Kailu |e verfasserin |4 aut | |
| 700 | 1 | |a Hao, Mengyue |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Yanjie |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Xinyu |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Zhifen |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Lijuan |e verfasserin |4 aut | |
| 700 | 1 | |a Chi, Xiaoyan |e verfasserin |4 aut | |
| 700 | 1 | |a Ge, Yihe |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Archives of microbiology |d 1974 |g 202(2020), 6 vom: 13. Aug., Seite 1507-1515 |w (DE-627)NLM000013242 |x 1432-072X |7 nnns |
| 773 | 1 | 8 | |g volume:202 |g year:2020 |g number:6 |g day:13 |g month:08 |g pages:1507-1515 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1007/s00203-020-01837-8 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 202 |j 2020 |e 6 |b 13 |c 08 |h 1507-1515 | ||