A metabolic network-based approach for developing feeding strategies for CHO cells to increase monoclonal antibody production
Chinese hamster ovary (CHO) cells are the main workhorse in the biopharmaceutical industry for the production of recombinant proteins, such as monoclonal antibodies. To date, a variety of metabolic engineering approaches have been used to improve the productivity of CHO cells. While genetic manipulations are potentially laborious in mammalian cells, rational design of CHO cell culture medium or efficient fed-batch strategies are more popular approaches for bioprocess optimization. In this study, a genome-scale metabolic network model of CHO cells was used to design feeding strategies for CHO cells to improve monoclonal antibody (mAb) production. A number of metabolites, including threonine and arachidonate, were suggested by the model to be added into cell culture medium. The designed composition has been experimentally validated, and then optimized, using design of experiment methods. About a two-fold increase in the total mAb expression has been observed using this strategy. Our approach can be used in similar bioprocess optimization problems, to suggest new ways of increasing production in different cell factories.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:43 |
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| Enthalten in: |
Bioprocess and biosystems engineering - 43(2020), 8 vom: 24. Aug., Seite 1381-1389 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Fouladiha, Hamideh [VerfasserIn] |
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| Links: |
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| Themen: |
Antibodies, Monoclonal |
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| Anmerkungen: |
Date Completed 09.06.2021 Date Revised 09.06.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1007/s00449-020-02332-6 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM307978494 |
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| 245 | 1 | 2 | |a A metabolic network-based approach for developing feeding strategies for CHO cells to increase monoclonal antibody production |
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| 520 | |a Chinese hamster ovary (CHO) cells are the main workhorse in the biopharmaceutical industry for the production of recombinant proteins, such as monoclonal antibodies. To date, a variety of metabolic engineering approaches have been used to improve the productivity of CHO cells. While genetic manipulations are potentially laborious in mammalian cells, rational design of CHO cell culture medium or efficient fed-batch strategies are more popular approaches for bioprocess optimization. In this study, a genome-scale metabolic network model of CHO cells was used to design feeding strategies for CHO cells to improve monoclonal antibody (mAb) production. A number of metabolites, including threonine and arachidonate, were suggested by the model to be added into cell culture medium. The designed composition has been experimentally validated, and then optimized, using design of experiment methods. About a two-fold increase in the total mAb expression has been observed using this strategy. Our approach can be used in similar bioprocess optimization problems, to suggest new ways of increasing production in different cell factories | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Central composite design | |
| 650 | 4 | |a Constrain-based modeling | |
| 650 | 4 | |a DoE | |
| 650 | 4 | |a Feeding strategies | |
| 650 | 4 | |a Metabolic network models | |
| 650 | 4 | |a Plackett–Burman | |
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| 650 | 7 | |a Culture Media |2 NLM | |
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| 700 | 1 | |a Marashi, Sayed-Amir |e verfasserin |4 aut | |
| 700 | 1 | |a Torkashvand, Fatemeh |e verfasserin |4 aut | |
| 700 | 1 | |a Mahboudi, Fereidoun |e verfasserin |4 aut | |
| 700 | 1 | |a Lewis, Nathan E |e verfasserin |4 aut | |
| 700 | 1 | |a Vaziri, Behrouz |e verfasserin |4 aut | |
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