Identification of a Second Type of AHL-lactonase from Rhodococcus sp. BH4, belonging to the α/β Hydrolase Superfamily
N-acyl-homoserine lactone (AHL)-mediated quorum sensing (QS) plays a major role in development of biofilms, which contribute to rise in infections and biofouling in water-related industries. Interference in QS, called quorum quenching (QQ), has recieved a lot of attention in recent years. Rhodococcus spp. are known to have prominent quorum quenching activity and in previous reports it was suggested that this genus possesses multiple QQ enzymes, but only one gene, qsdA, which encodes an AHL-lactonase belonging to phosphotriesterase family, has been identified. Therefore, we conducted a whole genome sequencing and analysis of Rhodococcus sp. BH4 isolated from a wastewater treatment plant. The sequencing revealed another gene encoding a QQ enzyme (named jydB) that exhibited a high AHL degrading activity. This QQ enzyme had a 46% amino acid sequence similarity with the AHL-lactonase (AidH) of Ochrobactrum sp. T63. HPLC analysis and AHL restoration experiments by acidification revealed that the jydB gene encodes an AHL-lactonase which shares the known characteristics of the α/β hydrolase family. Purified recombinant JydB demonstrated a high hydrolytic activity against various AHLs. Kinetic analysis of JydB revealed a high catalytic efficiency (kcat/KM) against C4-HSL and 3-oxo-C6 HSL, ranging from 1.88 × 106 to 1.45 × 106 M-1 s-1, with distinctly low KM values (0.16 - 0.24 mM). This study affirms that the AHL degrading activity and biofilm inhibition ability of Rhodococcus sp. BH4 may be due to the presence of multiple quorum quenching enzymes, including two types of AHL-lactonases, in addition to AHL-acylase and oxidoreductase, for which the genes have yet to be described.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2020 |
|---|---|
| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:30 |
|---|---|
| Enthalten in: |
Journal of microbiology and biotechnology - 30(2020), 6 vom: 28. Juni, Seite 937-945 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Ryu, Du-Hwan [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 08.10.2020 Date Revised 14.12.2022 published: Print Citation Status MEDLINE |
|---|
| doi: |
10.4014/jmb.2001.01006 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM307488942 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM307488942 | ||
| 003 | DE-627 | ||
| 005 | 20231225125654.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.4014/jmb.2001.01006 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1024.xml |
| 035 | |a (DE-627)NLM307488942 | ||
| 035 | |a (NLM)32160697 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Ryu, Du-Hwan |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Identification of a Second Type of AHL-lactonase from Rhodococcus sp. BH4, belonging to the α/β Hydrolase Superfamily |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 08.10.2020 | ||
| 500 | |a Date Revised 14.12.2022 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a N-acyl-homoserine lactone (AHL)-mediated quorum sensing (QS) plays a major role in development of biofilms, which contribute to rise in infections and biofouling in water-related industries. Interference in QS, called quorum quenching (QQ), has recieved a lot of attention in recent years. Rhodococcus spp. are known to have prominent quorum quenching activity and in previous reports it was suggested that this genus possesses multiple QQ enzymes, but only one gene, qsdA, which encodes an AHL-lactonase belonging to phosphotriesterase family, has been identified. Therefore, we conducted a whole genome sequencing and analysis of Rhodococcus sp. BH4 isolated from a wastewater treatment plant. The sequencing revealed another gene encoding a QQ enzyme (named jydB) that exhibited a high AHL degrading activity. This QQ enzyme had a 46% amino acid sequence similarity with the AHL-lactonase (AidH) of Ochrobactrum sp. T63. HPLC analysis and AHL restoration experiments by acidification revealed that the jydB gene encodes an AHL-lactonase which shares the known characteristics of the α/β hydrolase family. Purified recombinant JydB demonstrated a high hydrolytic activity against various AHLs. Kinetic analysis of JydB revealed a high catalytic efficiency (kcat/KM) against C4-HSL and 3-oxo-C6 HSL, ranging from 1.88 × 106 to 1.45 × 106 M-1 s-1, with distinctly low KM values (0.16 - 0.24 mM). This study affirms that the AHL degrading activity and biofilm inhibition ability of Rhodococcus sp. BH4 may be due to the presence of multiple quorum quenching enzymes, including two types of AHL-lactonases, in addition to AHL-acylase and oxidoreductase, for which the genes have yet to be described | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a AHL | |
| 650 | 4 | |a AHL-lactonase | |
| 650 | 4 | |a Quorum quenching | |
| 650 | 4 | |a Rhodococcus spp. | |
| 650 | 4 | |a biofilm | |
| 650 | 4 | |a quorum sensing | |
| 650 | 7 | |a N-butyrylhomoserine lactone |2 NLM | |
| 650 | 7 | |a Waste Water |2 NLM | |
| 650 | 7 | |a Carboxylic Ester Hydrolases |2 NLM | |
| 650 | 7 | |a EC 3.1.1.- |2 NLM | |
| 650 | 7 | |a N-acyl homoserine lactonase |2 NLM | |
| 650 | 7 | |a EC 3.1.1.- |2 NLM | |
| 650 | 7 | |a 4-Butyrolactone |2 NLM | |
| 650 | 7 | |a OL659KIY4X |2 NLM | |
| 700 | 1 | |a Lee, Sang-Won |e verfasserin |4 aut | |
| 700 | 1 | |a Mikolaityte, Viktorija |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Yea-Won |e verfasserin |4 aut | |
| 700 | 1 | |a Jeong, Hae Young |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Sang Jun |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Chung-Hak |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Jung-Kee |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of microbiology and biotechnology |d 1997 |g 30(2020), 6 vom: 28. Juni, Seite 937-945 |w (DE-627)NLM093752350 |x 1738-8872 |7 nnns |
| 773 | 1 | 8 | |g volume:30 |g year:2020 |g number:6 |g day:28 |g month:06 |g pages:937-945 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.4014/jmb.2001.01006 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 30 |j 2020 |e 6 |b 28 |c 06 |h 937-945 | ||