Longitudinal dynamics of the human B cell response to the yellow fever 17D vaccine
Copyright © 2020 the Author(s). Published by PNAS..
A comprehensive understanding of the development and evolution of human B cell responses induced by pathogen exposure will facilitate the design of next-generation vaccines. Here, we utilized a high-throughput single B cell cloning technology to longitudinally track the human B cell response to the yellow fever virus 17D (YFV-17D) vaccine. The early memory B cell (MBC) response was mediated by both classical immunoglobulin M (IgM) (IgM+CD27+) and switched immunoglobulin (swIg+) MBC populations; however, classical IgM MBCs waned rapidly, whereas swIg+ and atypical IgM+ and IgD+ MBCs were stable over time. Affinity maturation continued for 6 to 9 mo following vaccination, providing evidence for the persistence of germinal center activity long after the period of active viral replication in peripheral blood. Finally, a substantial fraction of the neutralizing antibody response was mediated by public clones that recognize a fusion loop-proximal antigenic site within domain II of the viral envelope glycoprotein. Overall, our findings provide a framework for understanding the dynamics and complexity of human B cell responses elicited by infection and vaccination.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:117 |
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| Enthalten in: |
Proceedings of the National Academy of Sciences of the United States of America - 117(2020), 12 vom: 24. März, Seite 6675-6685 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Wec, Anna Z [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 07.07.2020 Date Revised 25.04.2024 published: Print-Electronic GENBANK: MN993290, MN993593 Citation Status MEDLINE |
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| doi: |
10.1073/pnas.1921388117 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM307404390 |
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| 100 | 1 | |a Wec, Anna Z |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Longitudinal dynamics of the human B cell response to the yellow fever 17D vaccine |
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| 500 | |a Date Revised 25.04.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a GENBANK: MN993290, MN993593 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2020 the Author(s). Published by PNAS. | ||
| 520 | |a A comprehensive understanding of the development and evolution of human B cell responses induced by pathogen exposure will facilitate the design of next-generation vaccines. Here, we utilized a high-throughput single B cell cloning technology to longitudinally track the human B cell response to the yellow fever virus 17D (YFV-17D) vaccine. The early memory B cell (MBC) response was mediated by both classical immunoglobulin M (IgM) (IgM+CD27+) and switched immunoglobulin (swIg+) MBC populations; however, classical IgM MBCs waned rapidly, whereas swIg+ and atypical IgM+ and IgD+ MBCs were stable over time. Affinity maturation continued for 6 to 9 mo following vaccination, providing evidence for the persistence of germinal center activity long after the period of active viral replication in peripheral blood. Finally, a substantial fraction of the neutralizing antibody response was mediated by public clones that recognize a fusion loop-proximal antigenic site within domain II of the viral envelope glycoprotein. Overall, our findings provide a framework for understanding the dynamics and complexity of human B cell responses elicited by infection and vaccination | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a B cell memory | |
| 650 | 4 | |a antibody responses | |
| 650 | 4 | |a antiviral vaccination | |
| 650 | 4 | |a monoclonal antibody | |
| 650 | 4 | |a yellow fever virus | |
| 650 | 7 | |a Antibodies, Viral |2 NLM | |
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| 650 | 7 | |a Vaccines, Attenuated |2 NLM | |
| 650 | 7 | |a Viral Envelope Proteins |2 NLM | |
| 650 | 7 | |a Yellow Fever Vaccine |2 NLM | |
| 700 | 1 | |a Haslwanter, Denise |e verfasserin |4 aut | |
| 700 | 1 | |a Abdiche, Yasmina N |e verfasserin |4 aut | |
| 700 | 1 | |a Shehata, Laila |e verfasserin |4 aut | |
| 700 | 1 | |a Pedreño-Lopez, Nuria |e verfasserin |4 aut | |
| 700 | 1 | |a Moyer, Crystal L |e verfasserin |4 aut | |
| 700 | 1 | |a Bornholdt, Zachary A |e verfasserin |4 aut | |
| 700 | 1 | |a Lilov, Asparouh |e verfasserin |4 aut | |
| 700 | 1 | |a Nett, Juergen H |e verfasserin |4 aut | |
| 700 | 1 | |a Jangra, Rohit K |e verfasserin |4 aut | |
| 700 | 1 | |a Brown, Michael |e verfasserin |4 aut | |
| 700 | 1 | |a Watkins, David I |e verfasserin |4 aut | |
| 700 | 1 | |a Ahlm, Clas |e verfasserin |4 aut | |
| 700 | 1 | |a Forsell, Mattias N |e verfasserin |4 aut | |
| 700 | 1 | |a Rey, Félix A |e verfasserin |4 aut | |
| 700 | 1 | |a Barba-Spaeth, Giovanna |e verfasserin |4 aut | |
| 700 | 1 | |a Chandran, Kartik |e verfasserin |4 aut | |
| 700 | 1 | |a Walker, Laura M |e verfasserin |4 aut | |
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