Construction and development of FimH lectin domain for rising immune response after injection by uropathogenic E. coli
Uropathogenic E. coli is one of the major agents of urinary tract infection. Today, no effective treatment or vaccine against this infection is exist. Accordingly, in the present study, a genetic constrruct for inducing of cellular immune system was designed. At first, fimH gene from E. coli 35218 was amplified using PCR. PCR product inserted into pET23a expression vector and the recombinant vector was analysed by sequencing. The vector was transformed to E. coli strain Origami and the protein was expressed under the 1 mM IPTG. FimH was purified with Ni-NTA column and the purified protein was used for immunization of BALB/c. Two weeks after the last injection, lymphocyte proliferation assay was carried out. In addition, IL-4 and IFN-γ cytokines, total antibody serum, IgG1 and IgG2a isotypes were quantified. Finally, protection ability of the vaccine in bladder and kidney infection of mice was evaluated.The results indicated that cellular immune response has a main protective role against UTI and FimH, as a vaccine candidate, significantly increase lymphocyte proliferation, IFN-γ response and total antibody amount. Immunization of mice with FimH conferred effective protection of kidney and bladder against urinary tract infection by uropathogenic E. coli (P< 0.002). It can be concluded that, the current FimH will be valuable for more trying to prepare a new vaccine against UTI.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:28 |
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| Enthalten in: |
Human antibodies - 28(2020), 2 vom: 20., Seite 169-178 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zandi, M [VerfasserIn] |
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| Links: |
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| Themen: |
147680-16-8 |
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| Anmerkungen: |
Date Completed 20.05.2021 Date Revised 20.05.2021 published: Print Citation Status MEDLINE |
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| doi: |
10.3233/HAB-200404 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM307083748 |
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| 245 | 1 | 0 | |a Construction and development of FimH lectin domain for rising immune response after injection by uropathogenic E. coli |
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| 500 | |a Date Revised 20.05.2021 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Uropathogenic E. coli is one of the major agents of urinary tract infection. Today, no effective treatment or vaccine against this infection is exist. Accordingly, in the present study, a genetic constrruct for inducing of cellular immune system was designed. At first, fimH gene from E. coli 35218 was amplified using PCR. PCR product inserted into pET23a expression vector and the recombinant vector was analysed by sequencing. The vector was transformed to E. coli strain Origami and the protein was expressed under the 1 mM IPTG. FimH was purified with Ni-NTA column and the purified protein was used for immunization of BALB/c. Two weeks after the last injection, lymphocyte proliferation assay was carried out. In addition, IL-4 and IFN-γ cytokines, total antibody serum, IgG1 and IgG2a isotypes were quantified. Finally, protection ability of the vaccine in bladder and kidney infection of mice was evaluated.The results indicated that cellular immune response has a main protective role against UTI and FimH, as a vaccine candidate, significantly increase lymphocyte proliferation, IFN-γ response and total antibody amount. Immunization of mice with FimH conferred effective protection of kidney and bladder against urinary tract infection by uropathogenic E. coli (P< 0.002). It can be concluded that, the current FimH will be valuable for more trying to prepare a new vaccine against UTI | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Urinary tract infection | |
| 650 | 4 | |a fimh | |
| 650 | 4 | |a mouse model | |
| 650 | 4 | |a uropathogenic Escherichia coli | |
| 650 | 7 | |a Adhesins, Escherichia coli |2 NLM | |
| 650 | 7 | |a Antibodies, Bacterial |2 NLM | |
| 650 | 7 | |a Lectins |2 NLM | |
| 650 | 7 | |a fimH protein, E coli |2 NLM | |
| 650 | 7 | |a Fimbriae Proteins |2 NLM | |
| 650 | 7 | |a 147680-16-8 |2 NLM | |
| 700 | 1 | |a Fallah Mehrabadi, J |e verfasserin |4 aut | |
| 700 | 1 | |a Mahdavi, M |e verfasserin |4 aut | |
| 700 | 1 | |a Irani, Sh |e verfasserin |4 aut | |
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