Proximity-enabled bidirectional enzymatic repairing amplification for ultrasensitive fluorescence sensing of adenosine triphosphate
Copyright © 2020 Elsevier B.V. All rights reserved..
A novel fluorescence sensing strategy for ultrasensitive and highly specific detection of adenosine triphosphate (ATP) has been developed by the combination of the proximity ligation assay with bidirectional enzymatic repairing amplification (BERA). The strategy relies on proximity binding-triggered the release of palindromic tail that initiates bidirectional cyclic enzymatic repairing amplification reaction with the aid of polymerase and two DNA repairing enzymes, uracil-DNA glycosylase (UDG) and endonuclease IV (Endo IV). A fluorescence-quenched hairpin probe with a palindromic tail at the 3' end is skillfully designed that functions as not only the recognition element, primer, and polymerization template for BERA but also the indicator for fluorescence signal output. On the basis of the amplification strategy, this biosensor displays excellent sensitivity and selectivity for ATP detection with an outstanding detection limit of 0.81 pM. Through simultaneously enhancing the target response signal value and reducing nonspecific background, this work deducted the background effect, and showed high sensitivity and reproducibility. Moreover, our biosensor also shows promising potential in real sample analysis. Therefore, the proximity-enabled BERA strategy indeed creates a simple and valuable fluorescence sensing platform for ATP identification and related disease diagnosis and biomedical research.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:1104 |
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| Enthalten in: |
Analytica chimica acta - 1104(2020) vom: 01. Apr., Seite 156-163 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Li, Shasha [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 25.11.2020 Date Revised 25.11.2020 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.aca.2020.01.006 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2020 Elsevier B.V. All rights reserved. | ||
| 520 | |a A novel fluorescence sensing strategy for ultrasensitive and highly specific detection of adenosine triphosphate (ATP) has been developed by the combination of the proximity ligation assay with bidirectional enzymatic repairing amplification (BERA). The strategy relies on proximity binding-triggered the release of palindromic tail that initiates bidirectional cyclic enzymatic repairing amplification reaction with the aid of polymerase and two DNA repairing enzymes, uracil-DNA glycosylase (UDG) and endonuclease IV (Endo IV). A fluorescence-quenched hairpin probe with a palindromic tail at the 3' end is skillfully designed that functions as not only the recognition element, primer, and polymerization template for BERA but also the indicator for fluorescence signal output. On the basis of the amplification strategy, this biosensor displays excellent sensitivity and selectivity for ATP detection with an outstanding detection limit of 0.81 pM. Through simultaneously enhancing the target response signal value and reducing nonspecific background, this work deducted the background effect, and showed high sensitivity and reproducibility. Moreover, our biosensor also shows promising potential in real sample analysis. Therefore, the proximity-enabled BERA strategy indeed creates a simple and valuable fluorescence sensing platform for ATP identification and related disease diagnosis and biomedical research | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Adenosine triphosphate | |
| 650 | 4 | |a Bidirectional enzymatic repairing amplification | |
| 650 | 4 | |a Fluorescence sensing | |
| 650 | 4 | |a Palindromic sequence | |
| 650 | 4 | |a Proximity ligation assay | |
| 650 | 7 | |a Fluorescent Dyes |2 NLM | |
| 650 | 7 | |a Adenosine Triphosphate |2 NLM | |
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| 700 | 1 | |a Liu, Su |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Jingfeng |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Yihan |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Rufeng |e verfasserin |4 aut | |
| 700 | 1 | |a Qu, Xiaonan |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Yu |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Jiadong |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Jinghua |e verfasserin |4 aut | |
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