Contributions of molecular techniques in the chronic phase of Chagas disease in the absence of treatment
Copyright © 2020 Elsevier España, S.L.U. and Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica. All rights reserved..
INTRODUCTION: The chronic phase of Chagas disease (CD) is characterised by a low and intermittent parasitaemia. The Polymerase Chain Reaction (PCR) presents a variable sensitivity in this stage limiting its use as a diagnostic tool. Despite this, the use of PCR in untreated patients can provide information on the parasite behaviour and its presence in peripheral blood.
METHODS: A timely real-time PCR determination was performed on a cohort of 495 untreated chronic CD patients. Also, a subcohort of 29 patients was followed-up by serial real-time PCR during a period from 8 to 12 months in which they could not have access to the treatment due to lack of supply.
RESULTS: The positive percentage of real-time PCR in our series was 42%. Nevertheless, real-time PCR positive results were significantly higher in patients with five years or less of residence in Spain (P=.041). The detection of DNA was not related to the existence of cardiac and/or gastrointestinal abnormalities. In the follow-up subgroup, real-time PCR was consistently positive in 13.8% of patients, consistently negative in 31%, and intermittent in 55.2%.
CONCLUSIONS: The different real-time PCR results regarding the time of residence suggests the possible relationship of external factors in the parasite presence in peripheral blood. On the other hand, specific host factors may be involved in the behaviour of parasitaemia over time.
| Errataetall: |
CommentIn: Enferm Infecc Microbiol Clin. 2020 Oct;38(8):353-355. - PMID 32912656 |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:38 |
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| Enthalten in: |
Enfermedades infecciosas y microbiologia clinica (English ed.) - 38(2020), 8 vom: 19. Okt., Seite 356-360 |
| Sprache: |
Englisch |
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| Weiterer Titel: |
Aportaciones de las técnicas moleculares en la fase crónica de la Enfermedad de Chagas en ausencia de tratamiento |
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| Beteiligte Personen: |
Sulleiro, Elena [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 23.08.2021 Date Revised 23.08.2021 published: Print-Electronic CommentIn: Enferm Infecc Microbiol Clin. 2020 Oct;38(8):353-355. - PMID 32912656 Citation Status MEDLINE |
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| doi: |
10.1016/j.eimc.2020.01.003 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 245 | 1 | 0 | |a Contributions of molecular techniques in the chronic phase of Chagas disease in the absence of treatment |
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| 500 | |a CommentIn: Enferm Infecc Microbiol Clin. 2020 Oct;38(8):353-355. - PMID 32912656 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2020 Elsevier España, S.L.U. and Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica. All rights reserved. | ||
| 520 | |a INTRODUCTION: The chronic phase of Chagas disease (CD) is characterised by a low and intermittent parasitaemia. The Polymerase Chain Reaction (PCR) presents a variable sensitivity in this stage limiting its use as a diagnostic tool. Despite this, the use of PCR in untreated patients can provide information on the parasite behaviour and its presence in peripheral blood | ||
| 520 | |a METHODS: A timely real-time PCR determination was performed on a cohort of 495 untreated chronic CD patients. Also, a subcohort of 29 patients was followed-up by serial real-time PCR during a period from 8 to 12 months in which they could not have access to the treatment due to lack of supply | ||
| 520 | |a RESULTS: The positive percentage of real-time PCR in our series was 42%. Nevertheless, real-time PCR positive results were significantly higher in patients with five years or less of residence in Spain (P=.041). The detection of DNA was not related to the existence of cardiac and/or gastrointestinal abnormalities. In the follow-up subgroup, real-time PCR was consistently positive in 13.8% of patients, consistently negative in 31%, and intermittent in 55.2% | ||
| 520 | |a CONCLUSIONS: The different real-time PCR results regarding the time of residence suggests the possible relationship of external factors in the parasite presence in peripheral blood. On the other hand, specific host factors may be involved in the behaviour of parasitaemia over time | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Chronic Chagas disease | |
| 650 | 4 | |a Enfermedad Chagas crónica | |
| 650 | 4 | |a Follow-up | |
| 650 | 4 | |a No tratados | |
| 650 | 4 | |a Non-endemic area | |
| 650 | 4 | |a Positivity rate | |
| 650 | 4 | |a Reacción en cadena de la polimerasa a tiempo real | |
| 650 | 4 | |a Real-time polymerase chain reaction | |
| 650 | 4 | |a Residence time | |
| 650 | 4 | |a Seguimiento | |
| 650 | 4 | |a Tasa de positividad | |
| 650 | 4 | |a Tiempo residencia | |
| 650 | 4 | |a Untreated patients | |
| 650 | 4 | |a Zona no endémica | |
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