Chromatin accessibility analysis reveals regulatory dynamics of developing human retina and hiPSC-derived retinal organoids
Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC)..
Retinal organoids (ROs) derived from human induced pluripotent stem cells (hiPSCs) provide potential opportunities for studying human retinal development and disorders; however, to what extent ROs recapitulate the epigenetic features of human retinal development is unknown. In this study, we systematically profiled chromatin accessibility and transcriptional dynamics over long-term human retinal and RO development. Our results showed that ROs recapitulated the human retinogenesis to a great extent, but divergent chromatin features were also discovered. We further reconstructed the transcriptional regulatory network governing human and RO retinogenesis in vivo. Notably, NFIB and THRA were identified as regulators in human retinal development. The chromatin modifications between developing human and mouse retina were also cross-analyzed. Notably, we revealed an enriched bivalent modification of H3K4me3 and H3K27me3 in human but not in murine retinogenesis, suggesting a more dedicated epigenetic regulation on human genome.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:6 |
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| Enthalten in: |
Science advances - 6(2020), 6 vom: 13. Feb., Seite eaay5247 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Xie, Haohuan [VerfasserIn] |
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| Links: |
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| Themen: |
Biomarkers |
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| Anmerkungen: |
Date Completed 19.10.2020 Date Revised 19.10.2020 published: Electronic-eCollection Citation Status MEDLINE |
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| doi: |
10.1126/sciadv.aay5247 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM306767775 |
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| 245 | 1 | 0 | |a Chromatin accessibility analysis reveals regulatory dynamics of developing human retina and hiPSC-derived retinal organoids |
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| 520 | |a Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). | ||
| 520 | |a Retinal organoids (ROs) derived from human induced pluripotent stem cells (hiPSCs) provide potential opportunities for studying human retinal development and disorders; however, to what extent ROs recapitulate the epigenetic features of human retinal development is unknown. In this study, we systematically profiled chromatin accessibility and transcriptional dynamics over long-term human retinal and RO development. Our results showed that ROs recapitulated the human retinogenesis to a great extent, but divergent chromatin features were also discovered. We further reconstructed the transcriptional regulatory network governing human and RO retinogenesis in vivo. Notably, NFIB and THRA were identified as regulators in human retinal development. The chromatin modifications between developing human and mouse retina were also cross-analyzed. Notably, we revealed an enriched bivalent modification of H3K4me3 and H3K27me3 in human but not in murine retinogenesis, suggesting a more dedicated epigenetic regulation on human genome | ||
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| 650 | 7 | |a Chromatin |2 NLM | |
| 700 | 1 | |a Zhang, Wen |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Mei |e verfasserin |4 aut | |
| 700 | 1 | |a Akhtar, Tasneem |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Young |e verfasserin |4 aut | |
| 700 | 1 | |a Yi, Wenyang |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Xiao |e verfasserin |4 aut | |
| 700 | 1 | |a Zuo, Zuqi |e verfasserin |4 aut | |
| 700 | 1 | |a Wei, Min |e verfasserin |4 aut | |
| 700 | 1 | |a Fang, Xin |e verfasserin |4 aut | |
| 700 | 1 | |a Yao, Ziqin |e verfasserin |4 aut | |
| 700 | 1 | |a Dong, Kai |e verfasserin |4 aut | |
| 700 | 1 | |a Zhong, Suijuan |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Qiang |e verfasserin |4 aut | |
| 700 | 1 | |a Shen, Yong |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Qian |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Xiaoqun |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Huan |e verfasserin |4 aut | |
| 700 | 1 | |a Bao, Jin |e verfasserin |4 aut | |
| 700 | 1 | |a Qu, Kun |e verfasserin |4 aut | |
| 700 | 1 | |a Xue, Tian |e verfasserin |4 aut | |
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