Targeted De Novo Centromere Formation in Drosophila Reveals Plasticity and Maintenance Potential of CENP-A Chromatin
Copyright © 2020 Elsevier Inc. All rights reserved..
Centromeres are essential for accurate chromosome segregation and are marked by centromere protein A (CENP-A) nucleosomes. Mis-targeted CENP-A chromatin has been shown to seed centromeres at non-centromeric DNA. However, the requirements for such de novo centromere formation and transmission in vivo remain unknown. Here, we employ Drosophila melanogaster and the LacI/lacO system to investigate the ability of targeted de novo centromeres to assemble and be inherited through development. De novo centromeres form efficiently at six distinct genomic locations, which include actively transcribed chromatin and heterochromatin, and cause widespread chromosomal instability. During tethering, de novo centromeres sometimes prevail, causing the loss of the endogenous centromere via DNA breaks and HP1-dependent epigenetic inactivation. Transient induction of de novo centromeres and chromosome healing in early embryogenesis show that, once established, these centromeres can be maintained through development. Our results underpin the ability of CENP-A chromatin to establish and sustain mitotic centromere function in Drosophila.
| Errataetall: |
CommentIn: Dev Cell. 2020 Feb 10;52(3):257-258. - PMID 32049036 |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:52 |
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| Enthalten in: |
Developmental cell - 52(2020), 3 vom: 10. Feb., Seite 379-394.e7 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Palladino, Jason [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 04.08.2020 Date Revised 14.05.2021 published: Print CommentIn: Dev Cell. 2020 Feb 10;52(3):257-258. - PMID 32049036 Citation Status MEDLINE |
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| doi: |
10.1016/j.devcel.2020.01.005 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Centromeres are essential for accurate chromosome segregation and are marked by centromere protein A (CENP-A) nucleosomes. Mis-targeted CENP-A chromatin has been shown to seed centromeres at non-centromeric DNA. However, the requirements for such de novo centromere formation and transmission in vivo remain unknown. Here, we employ Drosophila melanogaster and the LacI/lacO system to investigate the ability of targeted de novo centromeres to assemble and be inherited through development. De novo centromeres form efficiently at six distinct genomic locations, which include actively transcribed chromatin and heterochromatin, and cause widespread chromosomal instability. During tethering, de novo centromeres sometimes prevail, causing the loss of the endogenous centromere via DNA breaks and HP1-dependent epigenetic inactivation. Transient induction of de novo centromeres and chromosome healing in early embryogenesis show that, once established, these centromeres can be maintained through development. Our results underpin the ability of CENP-A chromatin to establish and sustain mitotic centromere function in Drosophila | ||
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| 700 | 1 | |a Sposato, Anthony |e verfasserin |4 aut | |
| 700 | 1 | |a Mason, Timothy D |e verfasserin |4 aut | |
| 700 | 1 | |a Mellone, Barbara G |e verfasserin |4 aut | |
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