Vitamin-D3 (α-1, 25(OH) 2D3) Protects Retinal Pigment Epithelium From Hyperoxic Insults
Purpose: Oxidative stress affects the retinal pigment epithelium (RPE) leading to development of vascular eye diseases. Cholecalciferol (VIT-D) is a known modulator of oxidative stress and angiogenesis. This in vitro study was carried out to evaluate the protective role of VIT-D on RPE cells incubated under hyperoxic conditions.
Methods: Cadaver primary RPE (PRPE) cells were cultured in hyperoxia (40% O2) with or without VIT-D (α-1, 25(OH) 2D3). The functional and physiological effects of PRPE cells with VIT-D treatment were analyzed using molecular and biochemical tools.
Results: Vascular signaling modulators, such as vascular endothelial growth factor (VEGF) and Notch, were reduced in hyperoxic conditions but significantly upregulated in the presence of VIT-D. Additionally, PRPE conditioned medium with VIT-D induced the tubulogenesis in primary human umbilical vein endothelial cells (HUVEC) cells. VIT-D supplementation restored phagocytosis and transmembrane potential in PRPE cells cultured under hyperoxia.
Conclusions: VIT-D protects RPE cells and promotes angiogenesis under hyperoxic insult. These findings may give impetus to the potential of VIT-D as a therapeutic agent in hyperoxia induced retinal vascular diseases.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:61 |
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| Enthalten in: |
Investigative ophthalmology & visual science - 61(2020), 2 vom: 07. Feb., Seite 4 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Murugeswari, Ponnalagu [VerfasserIn] |
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| Links: |
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| Themen: |
1C6V77QF41 |
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| Anmerkungen: |
Date Completed 13.04.2020 Date Revised 08.07.2020 published: Print Citation Status MEDLINE |
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| doi: |
10.1167/iovs.61.2.4 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM306261952 |
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| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Purpose: Oxidative stress affects the retinal pigment epithelium (RPE) leading to development of vascular eye diseases. Cholecalciferol (VIT-D) is a known modulator of oxidative stress and angiogenesis. This in vitro study was carried out to evaluate the protective role of VIT-D on RPE cells incubated under hyperoxic conditions | ||
| 520 | |a Methods: Cadaver primary RPE (PRPE) cells were cultured in hyperoxia (40% O2) with or without VIT-D (α-1, 25(OH) 2D3). The functional and physiological effects of PRPE cells with VIT-D treatment were analyzed using molecular and biochemical tools | ||
| 520 | |a Results: Vascular signaling modulators, such as vascular endothelial growth factor (VEGF) and Notch, were reduced in hyperoxic conditions but significantly upregulated in the presence of VIT-D. Additionally, PRPE conditioned medium with VIT-D induced the tubulogenesis in primary human umbilical vein endothelial cells (HUVEC) cells. VIT-D supplementation restored phagocytosis and transmembrane potential in PRPE cells cultured under hyperoxia | ||
| 520 | |a Conclusions: VIT-D protects RPE cells and promotes angiogenesis under hyperoxic insult. These findings may give impetus to the potential of VIT-D as a therapeutic agent in hyperoxia induced retinal vascular diseases | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Receptors, Notch |2 NLM | |
| 650 | 7 | |a Vascular Endothelial Growth Factor A |2 NLM | |
| 650 | 7 | |a Vitamins |2 NLM | |
| 650 | 7 | |a Cholecalciferol |2 NLM | |
| 650 | 7 | |a 1C6V77QF41 |2 NLM | |
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| 700 | 1 | |a Krishna, Lekshmi |e verfasserin |4 aut | |
| 700 | 1 | |a Anandula, Venkata Ramana |e verfasserin |4 aut | |
| 700 | 1 | |a Jeyabalan, Nallathambi |e verfasserin |4 aut | |
| 700 | 1 | |a Chevour, Priyanka |e verfasserin |4 aut | |
| 700 | 1 | |a Jayadev, Chaitra |e verfasserin |4 aut | |
| 700 | 1 | |a Shetty, Rohit |e verfasserin |4 aut | |
| 700 | 1 | |a Carpentier, Gilles |e verfasserin |4 aut | |
| 700 | 1 | |a Kumaramanickavel, Govindaswamy |e verfasserin |4 aut | |
| 700 | 1 | |a Ghosh, Arkasubhra |e verfasserin |4 aut | |
| 700 | 1 | |a Das, Debashish |e verfasserin |4 aut | |
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