Constitutively expressed MHC class Ib molecules regulate macrophage M2b polarization and sepsis severity in irradiated mice
©2020 Society for Leukocyte Biology..
Macrophages can change their physiology in response to microenvironmental signals. This differentiation into classically activated M1 or alternatively activated M2 macrophages is known as polarization. In this study, we isolated bone marrow-derived macrophages from β2m-deficient (deficient in both MHC class Ia and Ib) and Kb Db -deficient (deficient only in MHC class Ia) mice and found that β2m-deficient macrophages showed a significantly lower M2b polarization efficiency. In addition, the absence of constitutive MHC class Ib expression decreased the stability of the Notch-1 intracellular domain. Finally, we found that β2m-deficient mice exposed to irradiation showed reduced bacterial translocation and sepsis severity. Overall, our study demonstrates that MHC class Ib molecules are essential for M2b macrophage polarization and suggests that MHC class Ib molecules play an important role during infection-induced innate immunity.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2020 |
|---|---|
| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:107 |
|---|---|
| Enthalten in: |
Journal of leukocyte biology - 107(2020), 3 vom: 15. März, Seite 445-453 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Yao, Yunliang [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Beta 2-Microglobulin |
|---|
| Anmerkungen: |
Date Completed 13.08.2020 Date Revised 13.08.2020 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1002/JLB.1AB1219-125RR |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM306126532 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM306126532 | ||
| 003 | DE-627 | ||
| 005 | 20231225122659.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1002/JLB.1AB1219-125RR |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1020.xml |
| 035 | |a (DE-627)NLM306126532 | ||
| 035 | |a (NLM)32017192 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Yao, Yunliang |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Constitutively expressed MHC class Ib molecules regulate macrophage M2b polarization and sepsis severity in irradiated mice |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 13.08.2020 | ||
| 500 | |a Date Revised 13.08.2020 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a ©2020 Society for Leukocyte Biology. | ||
| 520 | |a Macrophages can change their physiology in response to microenvironmental signals. This differentiation into classically activated M1 or alternatively activated M2 macrophages is known as polarization. In this study, we isolated bone marrow-derived macrophages from β2m-deficient (deficient in both MHC class Ia and Ib) and Kb Db -deficient (deficient only in MHC class Ia) mice and found that β2m-deficient macrophages showed a significantly lower M2b polarization efficiency. In addition, the absence of constitutive MHC class Ib expression decreased the stability of the Notch-1 intracellular domain. Finally, we found that β2m-deficient mice exposed to irradiation showed reduced bacterial translocation and sepsis severity. Overall, our study demonstrates that MHC class Ib molecules are essential for M2b macrophage polarization and suggests that MHC class Ib molecules play an important role during infection-induced innate immunity | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a M2b | |
| 650 | 4 | |a MHC class Ib | |
| 650 | 4 | |a irradiation-associated sepsis | |
| 650 | 4 | |a macrophage polarization | |
| 650 | 7 | |a Histocompatibility Antigens Class I |2 NLM | |
| 650 | 7 | |a beta 2-Microglobulin |2 NLM | |
| 700 | 1 | |a Zhang, Ting |e verfasserin |4 aut | |
| 700 | 1 | |a Ru, Xiaochen |e verfasserin |4 aut | |
| 700 | 1 | |a Qian, Jing |e verfasserin |4 aut | |
| 700 | 1 | |a Tong, Zhaowei |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Xiaoyu |e verfasserin |4 aut | |
| 700 | 1 | |a Kong, Xiangyang |e verfasserin |4 aut | |
| 700 | 1 | |a Yao, Wenjia |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Hongchang |e verfasserin |4 aut | |
| 700 | 1 | |a Zhong, Jianfeng |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of leukocyte biology |d 1986 |g 107(2020), 3 vom: 15. März, Seite 445-453 |w (DE-627)NLM012617644 |x 1938-3673 |7 nnns |
| 773 | 1 | 8 | |g volume:107 |g year:2020 |g number:3 |g day:15 |g month:03 |g pages:445-453 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1002/JLB.1AB1219-125RR |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 107 |j 2020 |e 3 |b 15 |c 03 |h 445-453 | ||