Transcriptome and proteome analyses reveal selenium mediated amelioration of arsenic toxicity in rice (Oryza sativa L.)
Copyright © 2020 Elsevier B.V. All rights reserved..
Arsenic (As), a chronic poison and non-threshold carcinogen, is a food chain contaminant in rice, posing yield losses as well as serious health risks. Selenium (Se), a trace element, is a known antagonist of As toxicity. In present study, RNA seq. and proteome profiling, along with morphological analyses were performed to explore molecular cross-talk involved in Se mediated As stress amelioration. The repair of As induced structural deformities involving disintegration of cell wall and membranes were observed upon Se supplementation. The expression of As transporter genes viz., NIP1;1, NIP2;1, ABCG5, NRAMP1, NRAMP5, TIP2;2 as well as sulfate transporters, SULTR3;1 and SULTR3;6, were higher in As + Se compared to As alone exposure, which resulted in reduced As accumulation and toxicity. The higher expression of regulatory elements like AUX/IAA, WRKY and MYB TFs during As + Se exposure was also observed. The up-regulation of GST, PRX and GRX during As + Se exposure confirmed the amelioration of As induced oxidative stress. The abundance of proteins involved in photosynthesis, energy metabolism, transport, signaling and ROS homeostasis were found higher in As + Se than in As alone exposure. Overall, present study identified Se responsive pathways, genes and proteins involved to cope-up with As toxicity in rice.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:390 |
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| Enthalten in: |
Journal of hazardous materials - 390(2020) vom: 15. Mai, Seite 122122 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Chauhan, Reshu [VerfasserIn] |
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| Links: |
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| Themen: |
Arsenic |
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| Anmerkungen: |
Date Completed 28.01.2021 Date Revised 28.01.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.jhazmat.2020.122122 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM306024853 |
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| 100 | 1 | |a Chauhan, Reshu |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Transcriptome and proteome analyses reveal selenium mediated amelioration of arsenic toxicity in rice (Oryza sativa L.) |
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| 500 | |a published: Print-Electronic | ||
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| 520 | |a Copyright © 2020 Elsevier B.V. All rights reserved. | ||
| 520 | |a Arsenic (As), a chronic poison and non-threshold carcinogen, is a food chain contaminant in rice, posing yield losses as well as serious health risks. Selenium (Se), a trace element, is a known antagonist of As toxicity. In present study, RNA seq. and proteome profiling, along with morphological analyses were performed to explore molecular cross-talk involved in Se mediated As stress amelioration. The repair of As induced structural deformities involving disintegration of cell wall and membranes were observed upon Se supplementation. The expression of As transporter genes viz., NIP1;1, NIP2;1, ABCG5, NRAMP1, NRAMP5, TIP2;2 as well as sulfate transporters, SULTR3;1 and SULTR3;6, were higher in As + Se compared to As alone exposure, which resulted in reduced As accumulation and toxicity. The higher expression of regulatory elements like AUX/IAA, WRKY and MYB TFs during As + Se exposure was also observed. The up-regulation of GST, PRX and GRX during As + Se exposure confirmed the amelioration of As induced oxidative stress. The abundance of proteins involved in photosynthesis, energy metabolism, transport, signaling and ROS homeostasis were found higher in As + Se than in As alone exposure. Overall, present study identified Se responsive pathways, genes and proteins involved to cope-up with As toxicity in rice | ||
| 650 | 4 | |a Journal Article | |
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| 700 | 1 | |a Indoliya, Yuvraj |e verfasserin |4 aut | |
| 700 | 1 | |a Chauhan, Abhishek Singh |e verfasserin |4 aut | |
| 700 | 1 | |a Mishra, Shashank |e verfasserin |4 aut | |
| 700 | 1 | |a Agrawal, Lalit |e verfasserin |4 aut | |
| 700 | 1 | |a Srivastava, Sudhakar |e verfasserin |4 aut | |
| 700 | 1 | |a Dwivedi, Sanjay |e verfasserin |4 aut | |
| 700 | 1 | |a Singh, Poonam C |e verfasserin |4 aut | |
| 700 | 1 | |a Mallick, Shekhar |e verfasserin |4 aut | |
| 700 | 1 | |a Chauhan, Puneet Singh |e verfasserin |4 aut | |
| 700 | 1 | |a Pande, Veena |e verfasserin |4 aut | |
| 700 | 1 | |a Chakrabarty, Debasis |e verfasserin |4 aut | |
| 700 | 1 | |a Tripathi, Rudra Deo |e verfasserin |4 aut | |
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