CRISPR/Cas12a-based biosensing platform for precise and efficient screening of CRISPR/Cas9-induced biallelic mutants
Copyright © 2019 Elsevier B.V. All rights reserved..
CRISPR/Cas9 is a robust tool to manipulate genes in a wide range of species. Although several methods are introduced to identify the CRISPR/Cas9-induced mutations, they are labor-intensive, costly, and not easy to use or were sequence-limited. Moreover, few of them could identify the biallelic mutants that are the desired outcomes of targeted mutagenesis. Recently, a CRISPR/Cas12a-mediated biosensing platform was developed to detect nucleic acids based on the collateral DNA cleavage activity of Cas12a; it was highly sensitive, specific, rapid, and cost-efficient for genotyping, mutation detection, and single nucleotide polymorphism (SNP) identification, thereby deeming it as an innovative method for screening the CRISPR/Cas9-induced biallelic mutants. Thus, the CRISPR/Cas12a-based biosensing platform has been successfully utilized for screening 23 CRISPR/Cas9-induced biallelic mutants in Thp-1 cells, which were also confirmed by direct sequencing and ELISA. The precision and efficiency of CRISPR/Cas12a-based biosensing platform make it a promising tool for screening of CRISPR/Cas9-induced biallelic mutants in the future.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:210 |
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| Enthalten in: |
Talanta - 210(2020) vom: 01. Apr., Seite 120613 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Xiao, Guohui [VerfasserIn] |
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| Links: |
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| Themen: |
63231-63-0 |
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| Anmerkungen: |
Date Completed 26.10.2020 Date Revised 26.10.2020 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.talanta.2019.120613 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a CRISPR/Cas9 is a robust tool to manipulate genes in a wide range of species. Although several methods are introduced to identify the CRISPR/Cas9-induced mutations, they are labor-intensive, costly, and not easy to use or were sequence-limited. Moreover, few of them could identify the biallelic mutants that are the desired outcomes of targeted mutagenesis. Recently, a CRISPR/Cas12a-mediated biosensing platform was developed to detect nucleic acids based on the collateral DNA cleavage activity of Cas12a; it was highly sensitive, specific, rapid, and cost-efficient for genotyping, mutation detection, and single nucleotide polymorphism (SNP) identification, thereby deeming it as an innovative method for screening the CRISPR/Cas9-induced biallelic mutants. Thus, the CRISPR/Cas12a-based biosensing platform has been successfully utilized for screening 23 CRISPR/Cas9-induced biallelic mutants in Thp-1 cells, which were also confirmed by direct sequencing and ELISA. The precision and efficiency of CRISPR/Cas12a-based biosensing platform make it a promising tool for screening of CRISPR/Cas9-induced biallelic mutants in the future | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Biallelic mutants | |
| 650 | 4 | |a CRISPR/Cas12a | |
| 650 | 4 | |a CRISPR/Cas9 | |
| 650 | 4 | |a Mutants screening | |
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| 700 | 1 | |a Liu, Houming |e verfasserin |4 aut | |
| 700 | 1 | |a He, Xing |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Su |e verfasserin |4 aut | |
| 700 | 1 | |a Liang, Zhihang |e verfasserin |4 aut | |
| 700 | 1 | |a Guo, Huixin |e verfasserin |4 aut | |
| 700 | 1 | |a Ou, Min |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Lin |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Lei |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Tianyu |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Guoliang |e verfasserin |4 aut | |
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