Biotin interferences : Have we neglected the impact on serological markers?
Copyright © 2020 Elsevier B.V. All rights reserved..
BACKGROUND: Biotin has been reported to be a leading cause of interference on several immunoassay platforms using the streptavidin-biotin immobilization system. While biotin interferences have now been well characterized for several assays, only few data are available on their impact on serological markers of infectious viral diseases.
METHODS: Overall, 10 healthy volunteers (HVs) received a single 100 mg dose of biotin to evaluate its effect on hepatitis B serological markers. Blood samples were taken several times before and after biotin intake. In addition, spiking experiments were applied to investigate biotin's impact on anti-HIV/p24 Ag and anti-HCV antibody levels. Several procedures designed to overcome this interference were evaluated.
RESULTS: Biotin intake resulted in a false-negative anti-HBs immunological status (<10 mIU/mL) in 40.0% of cases. According to our anti-HBc and anti-HBe results, biotin intake was associated with 90.0% and 80.0% of false positive results, respectively. At the theoretical biotin peak concentration following a 100 mg intake, 50.0% and 66.6% of anti-HIV and anti-HCV results were false negatives, respectively. All the procedures evaluated to overcome the interference were proven effective.
CONCLUSION: HBV, HCV, and HIV serological markers are likely to be highly sensitive to biotin. Our data confirm that the scope of biotin interference is broader than commonly described.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:503 |
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| Enthalten in: |
Clinica chimica acta; international journal of clinical chemistry - 503(2020) vom: 02. Apr., Seite 107-112 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Bayart, Jean-Louis [VerfasserIn] |
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| Links: |
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| Themen: |
6SO6U10H04 |
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| Anmerkungen: |
Date Completed 23.09.2020 Date Revised 23.09.2020 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.cca.2020.01.012 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM305751042 |
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| 100 | 1 | |a Bayart, Jean-Louis |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Biotin interferences |b Have we neglected the impact on serological markers? |
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| 500 | |a Date Revised 23.09.2020 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2020 Elsevier B.V. All rights reserved. | ||
| 520 | |a BACKGROUND: Biotin has been reported to be a leading cause of interference on several immunoassay platforms using the streptavidin-biotin immobilization system. While biotin interferences have now been well characterized for several assays, only few data are available on their impact on serological markers of infectious viral diseases | ||
| 520 | |a METHODS: Overall, 10 healthy volunteers (HVs) received a single 100 mg dose of biotin to evaluate its effect on hepatitis B serological markers. Blood samples were taken several times before and after biotin intake. In addition, spiking experiments were applied to investigate biotin's impact on anti-HIV/p24 Ag and anti-HCV antibody levels. Several procedures designed to overcome this interference were evaluated | ||
| 520 | |a RESULTS: Biotin intake resulted in a false-negative anti-HBs immunological status (<10 mIU/mL) in 40.0% of cases. According to our anti-HBc and anti-HBe results, biotin intake was associated with 90.0% and 80.0% of false positive results, respectively. At the theoretical biotin peak concentration following a 100 mg intake, 50.0% and 66.6% of anti-HIV and anti-HCV results were false negatives, respectively. All the procedures evaluated to overcome the interference were proven effective | ||
| 520 | |a CONCLUSION: HBV, HCV, and HIV serological markers are likely to be highly sensitive to biotin. Our data confirm that the scope of biotin interference is broader than commonly described | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a AIDS | |
| 650 | 4 | |a Biotin | |
| 650 | 4 | |a Hepatitis | |
| 650 | 4 | |a Interference | |
| 650 | 4 | |a Serology | |
| 650 | 4 | |a Virology | |
| 650 | 7 | |a Antibodies, Viral |2 NLM | |
| 650 | 7 | |a Biomarkers |2 NLM | |
| 650 | 7 | |a Biotin |2 NLM | |
| 650 | 7 | |a 6SO6U10H04 |2 NLM | |
| 700 | 1 | |a Favresse, Julien |e verfasserin |4 aut | |
| 700 | 1 | |a Stoefs, Anke |e verfasserin |4 aut | |
| 700 | 1 | |a Closset, Mélanie |e verfasserin |4 aut | |
| 700 | 1 | |a Roy, Tatiana |e verfasserin |4 aut | |
| 700 | 1 | |a Fillée, Catherine |e verfasserin |4 aut | |
| 700 | 1 | |a Rodriguez-Villalobos, Hector |e verfasserin |4 aut | |
| 700 | 1 | |a Kabamba-Mukadi, Benoît |e verfasserin |4 aut | |
| 700 | 1 | |a Gruson, Damien |e verfasserin |4 aut | |
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