Hemolytic specimens in complete blood cell count : Red cell parameters could be revised by plasma free hemoglobin
© 2020 The Authors. Journal of Clinical Laboratory Analysis Published by Wiley Periodicals, Inc..
INTRODUCTION: Hemolysis is the main cause of unqualified clinical samples. In this study, we established a method for detecting and evaluating hemolysis in whole blood test. We used a mathematical formula for correcting the influence of hemolysis on complete blood cell count (CBC) so as to avoid re-venipuncture and obtain more accurate parameters of red blood cell detection, reduce the burden of patients, and improve the efficiency of diagnosis and treatment.
METHODS: Hemolytic samples were selected and then corrected using the new formula. Plasma free hemoglobin (fHB) was used as the criterion to determine the degree of hemolysis; the uncertainty of measurement is acceptable as the limit value of deviation between the measured value and the revised value. Hemolysis simulation analysis in vitro and continuous monitoring of clinical patients were used to verify the correction effect.
RESULTS: A total of 83 clinical samples with hemolysis were collected and analyzed; fHB 1.4 g/L was selected as the unacceptable value for clinical hemolysis detection. In hemolytic samples, the red blood cell parameters corrected by formula are significantly different from those uncorrected and had a good consistency with those before hemolysis.
CONCLUSION: The results show that the hemolysis phenomenon of CBC has a significant impact on routine blood testing. By using the new formula, the influence of hemolysis on erythrocyte and related parameters can be quickly and easily corrected, thus avoiding venipuncture again for re-examination, reducing diagnostic errors, and saving medical resources.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:34 |
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| Enthalten in: |
Journal of clinical laboratory analysis - 34(2020), 6 vom: 02. Juni, Seite e23218 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Peng, Zhaoyang [VerfasserIn] |
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| Links: |
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| Themen: |
Blood cell count |
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| Anmerkungen: |
Date Completed 19.05.2021 Date Revised 19.05.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1002/jcla.23218 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM305654519 |
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| 500 | |a Date Completed 19.05.2021 | ||
| 500 | |a Date Revised 19.05.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2020 The Authors. Journal of Clinical Laboratory Analysis Published by Wiley Periodicals, Inc. | ||
| 520 | |a INTRODUCTION: Hemolysis is the main cause of unqualified clinical samples. In this study, we established a method for detecting and evaluating hemolysis in whole blood test. We used a mathematical formula for correcting the influence of hemolysis on complete blood cell count (CBC) so as to avoid re-venipuncture and obtain more accurate parameters of red blood cell detection, reduce the burden of patients, and improve the efficiency of diagnosis and treatment | ||
| 520 | |a METHODS: Hemolytic samples were selected and then corrected using the new formula. Plasma free hemoglobin (fHB) was used as the criterion to determine the degree of hemolysis; the uncertainty of measurement is acceptable as the limit value of deviation between the measured value and the revised value. Hemolysis simulation analysis in vitro and continuous monitoring of clinical patients were used to verify the correction effect | ||
| 520 | |a RESULTS: A total of 83 clinical samples with hemolysis were collected and analyzed; fHB 1.4 g/L was selected as the unacceptable value for clinical hemolysis detection. In hemolytic samples, the red blood cell parameters corrected by formula are significantly different from those uncorrected and had a good consistency with those before hemolysis | ||
| 520 | |a CONCLUSION: The results show that the hemolysis phenomenon of CBC has a significant impact on routine blood testing. By using the new formula, the influence of hemolysis on erythrocyte and related parameters can be quickly and easily corrected, thus avoiding venipuncture again for re-examination, reducing diagnostic errors, and saving medical resources | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a blood cell count | |
| 650 | 4 | |a diagnostic errors | |
| 650 | 4 | |a free hemoglobin | |
| 650 | 4 | |a hemolysis | |
| 650 | 7 | |a Hemoglobins |2 NLM | |
| 700 | 1 | |a Xiang, Wenqing |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Jianming |e verfasserin |4 aut | |
| 700 | 1 | |a Cao, Jiajia |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Zhe |e verfasserin |4 aut | |
| 700 | 1 | |a Gao, Hui |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Junfeng |e verfasserin |4 aut | |
| 700 | 1 | |a Shen, Hongqiang |e verfasserin |4 aut | |
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