Precursors for Nonlymphoid-Tissue Treg Cells Reside in Secondary Lymphoid Organs and Are Programmed by the Transcription Factor BATF
Copyright © 2019 The Author(s). Published by Elsevier Inc. All rights reserved..
Specialized regulatory T (Treg) cells accumulate and perform homeostatic and regenerative functions in nonlymphoid tissues. Whether common precursors for nonlymphoid-tissue Treg cells exist and how they differentiate remain elusive. Using transcription factor nuclear factor, interleukin 3 regulated (Nfil3) reporter mice and single-cell RNA-sequencing (scRNA-seq), we identified two precursor stages of interleukin 33 (IL-33) receptor ST2-expressing nonlymphoid tissue Treg cells, which resided in the spleen and lymph nodes. Global chromatin profiling of nonlymphoid tissue Treg cells and the two precursor stages revealed a stepwise acquisition of chromatin accessibility and reprogramming toward the nonlymphoid-tissue Treg cell phenotype. Mechanistically, we identified and validated the transcription factor Batf as the driver of the molecular tissue program in the precursors. Understanding this tissue development program will help to harness regenerative properties of tissue Treg cells for therapy.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:52 |
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| Enthalten in: |
Immunity - 52(2020), 2 vom: 18. Feb., Seite 295-312.e11 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Delacher, Michael [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 21.04.2020 Date Revised 10.01.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.immuni.2019.12.002 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 500 | |a Date Revised 10.01.2021 | ||
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| 520 | |a Copyright © 2019 The Author(s). Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a Specialized regulatory T (Treg) cells accumulate and perform homeostatic and regenerative functions in nonlymphoid tissues. Whether common precursors for nonlymphoid-tissue Treg cells exist and how they differentiate remain elusive. Using transcription factor nuclear factor, interleukin 3 regulated (Nfil3) reporter mice and single-cell RNA-sequencing (scRNA-seq), we identified two precursor stages of interleukin 33 (IL-33) receptor ST2-expressing nonlymphoid tissue Treg cells, which resided in the spleen and lymph nodes. Global chromatin profiling of nonlymphoid tissue Treg cells and the two precursor stages revealed a stepwise acquisition of chromatin accessibility and reprogramming toward the nonlymphoid-tissue Treg cell phenotype. Mechanistically, we identified and validated the transcription factor Batf as the driver of the molecular tissue program in the precursors. Understanding this tissue development program will help to harness regenerative properties of tissue Treg cells for therapy | ||
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| 700 | 1 | |a Mallm, Jan-Philipp |e verfasserin |4 aut | |
| 700 | 1 | |a Bauer, Katharina |e verfasserin |4 aut | |
| 700 | 1 | |a Simon, Malte |e verfasserin |4 aut | |
| 700 | 1 | |a Riegel, Dania |e verfasserin |4 aut | |
| 700 | 1 | |a Rendeiro, André F |e verfasserin |4 aut | |
| 700 | 1 | |a Bittner, Sebastian |e verfasserin |4 aut | |
| 700 | 1 | |a Sanderink, Lieke |e verfasserin |4 aut | |
| 700 | 1 | |a Pant, Asmita |e verfasserin |4 aut | |
| 700 | 1 | |a Schmidleithner, Lisa |e verfasserin |4 aut | |
| 700 | 1 | |a Braband, Kathrin L |e verfasserin |4 aut | |
| 700 | 1 | |a Echtenachter, Bernd |e verfasserin |4 aut | |
| 700 | 1 | |a Fischer, Alexander |e verfasserin |4 aut | |
| 700 | 1 | |a Giunchiglia, Valentina |e verfasserin |4 aut | |
| 700 | 1 | |a Hoffmann, Petra |e verfasserin |4 aut | |
| 700 | 1 | |a Edinger, Matthias |e verfasserin |4 aut | |
| 700 | 1 | |a Bock, Christoph |e verfasserin |4 aut | |
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| 700 | 1 | |a Feuerer, Markus |e verfasserin |4 aut | |
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