MiR-27b suppresses epithelial-mesenchymal transition and chemoresistance in lung cancer by targeting Snail1
Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved..
HEADING AIMS: MicroRNA-27b (miR-27b) has been shown to play a role in the progression of many different forms of cancer, but its specific relevance in the context of non-small cell lung cancer (NSCLC) remains uncertain. As such, this study sought to explore the role of miR-27b in NSCLC and the mechanisms whereby it functions.
MATERIALS AND METHODS: We quantified miR-27b and target gene expression via quantitative real-time PCR (RT-qPCR).We then used functional including proliferation assays, migration assay, flow cytometry, and western blotting to explore the mechanisms whereby miR-27b functions in vitro and in vivo. We additionally confirmed miR-27b target genes via luciferase reporter assay.
KEY FINDINGS: We observed a marked decrease in miR-27b expression in NSCLC patient samples relative to paracancerous control tissues. We further found that altering miR-27b expression levels in vitro affected NSCLC tumor cell migration, proliferation, and ability to undergo epithelial-mesenchymal transition. Through the use of target prediction algorithms we identified Snail to be a miR-27b target protein that was suppressed when this miRNA was highlight expressed. Lastly, we found miR-27b expression to increase NSCLC cell sensitivity to cisplatin through its ability to target Snail.
SIGNIFICANCE: Our results clearly demonstrate that miR-27b can suppress NSCLC tumor development and progression, highlighting this miR-27b/Snail1 axis as putative target for the therapeutic treatment of NSCLC.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2020 |
|---|---|
| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:254 |
|---|---|
| Enthalten in: |
Life sciences - 254(2020) vom: 01. Aug., Seite 117238 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Zhang, Jun [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Antineoplastic Agents |
|---|
| Anmerkungen: |
Date Completed 21.07.2020 Date Revised 15.04.2022 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.lfs.2019.117238 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM304872369 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM304872369 | ||
| 003 | DE-627 | ||
| 005 | 20231225115923.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.lfs.2019.117238 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1016.xml |
| 035 | |a (DE-627)NLM304872369 | ||
| 035 | |a (NLM)31887300 | ||
| 035 | |a (PII)S0024-3205(19)31166-X | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Zhang, Jun |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a MiR-27b suppresses epithelial-mesenchymal transition and chemoresistance in lung cancer by targeting Snail1 |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 21.07.2020 | ||
| 500 | |a Date Revised 15.04.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a HEADING AIMS: MicroRNA-27b (miR-27b) has been shown to play a role in the progression of many different forms of cancer, but its specific relevance in the context of non-small cell lung cancer (NSCLC) remains uncertain. As such, this study sought to explore the role of miR-27b in NSCLC and the mechanisms whereby it functions | ||
| 520 | |a MATERIALS AND METHODS: We quantified miR-27b and target gene expression via quantitative real-time PCR (RT-qPCR).We then used functional including proliferation assays, migration assay, flow cytometry, and western blotting to explore the mechanisms whereby miR-27b functions in vitro and in vivo. We additionally confirmed miR-27b target genes via luciferase reporter assay | ||
| 520 | |a KEY FINDINGS: We observed a marked decrease in miR-27b expression in NSCLC patient samples relative to paracancerous control tissues. We further found that altering miR-27b expression levels in vitro affected NSCLC tumor cell migration, proliferation, and ability to undergo epithelial-mesenchymal transition. Through the use of target prediction algorithms we identified Snail to be a miR-27b target protein that was suppressed when this miRNA was highlight expressed. Lastly, we found miR-27b expression to increase NSCLC cell sensitivity to cisplatin through its ability to target Snail | ||
| 520 | |a SIGNIFICANCE: Our results clearly demonstrate that miR-27b can suppress NSCLC tumor development and progression, highlighting this miR-27b/Snail1 axis as putative target for the therapeutic treatment of NSCLC | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Chemoresistance | |
| 650 | 4 | |a Lung cancer | |
| 650 | 4 | |a Snail1 | |
| 650 | 4 | |a miR-27b | |
| 650 | 7 | |a Antineoplastic Agents |2 NLM | |
| 650 | 7 | |a MIRN27 microRNA, human |2 NLM | |
| 650 | 7 | |a MicroRNAs |2 NLM | |
| 650 | 7 | |a SNAI1 protein, human |2 NLM | |
| 650 | 7 | |a Snail Family Transcription Factors |2 NLM | |
| 700 | 1 | |a Hua, Xionghuai |e verfasserin |4 aut | |
| 700 | 1 | |a Qi, Na |e verfasserin |4 aut | |
| 700 | 1 | |a Han, Guangsen |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Juan |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Yongkui |e verfasserin |4 aut | |
| 700 | 1 | |a Wei, Xiufeng |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Haomiao |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Xiankai |e verfasserin |4 aut | |
| 700 | 1 | |a Leng, Changsen |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Qi |e verfasserin |4 aut | |
| 700 | 1 | |a Lu, Yingmin |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Yin |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Life sciences |d 1965 |g 254(2020) vom: 01. Aug., Seite 117238 |w (DE-627)NLM000007579 |x 1879-0631 |7 nnns |
| 773 | 1 | 8 | |g volume:254 |g year:2020 |g day:01 |g month:08 |g pages:117238 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.lfs.2019.117238 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 254 |j 2020 |b 01 |c 08 |h 117238 | ||