Evaluation of the pol/S Gene Overlapping Mutations in Chronic Hepatitis B Patients in Northern Cyprus
Mutations associated with the pol and the S gene can emerge as a consequence of the high replication capacity and proofreading deficiencies of hepatitis B virus during replication. The current study was constructed to evaluate primary, partial, compensatory and the escape mutations in chronic hepatitis B patients in Northern Cyprus. The samples of HBsAg positive treatment naïve 100 patients were involved in this study. HBV pol gene region was sequenced, amplified and HBV pol/S gene mutations were determined. The samples of thirty-two patients were excluded because of their low viral load (HBV < 1000 ıu/ml). Among the sequenced 68 samples, there was a partial mutation (1.5%) and 36.7% displayed a resistance profile to lamivudine, adevofir, and telbivudine. Immune response escape, vaccine escape, HBIg and diagnosis escape mutations were determined in 24%, 10%, 6%, and 4% samples of the patients, respectively. Additionally, there were six different combined mutations. These data underscored that there is no concern for primary mutations in Northern Cyprus, however, we have identified a compensatory mutation (rtV173M) that may have primary mutation characteristics by combining with other mutation patterns. Additionally, HBsAg escape mutants demonstrated that detection of the S gene together with the pol gene mutations might be beneficial and important to monitor the surveillance of S variants.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2019 |
---|---|
Erschienen: |
2019 |
Enthalten in: |
Zur Gesamtaufnahme - volume:68 |
---|---|
Enthalten in: |
Polish journal of microbiology - 68(2019), 3 vom: 24. Sept., Seite 317-322 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Arikan, Ayse [VerfasserIn] |
---|
Links: |
---|
Themen: |
2T8Q726O95 |
---|
Anmerkungen: |
Date Completed 14.01.2020 Date Revised 04.06.2020 published: Print-Electronic Citation Status MEDLINE |
---|
doi: |
10.33073/pjm-2019-034 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM304808938 |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | NLM304808938 | ||
003 | DE-627 | ||
005 | 20231225115801.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231225s2019 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.33073/pjm-2019-034 |2 doi | |
028 | 5 | 2 | |a pubmed24n1016.xml |
035 | |a (DE-627)NLM304808938 | ||
035 | |a (NLM)31880877 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Arikan, Ayse |e verfasserin |4 aut | |
245 | 1 | 0 | |a Evaluation of the pol/S Gene Overlapping Mutations in Chronic Hepatitis B Patients in Northern Cyprus |
264 | 1 | |c 2019 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 14.01.2020 | ||
500 | |a Date Revised 04.06.2020 | ||
500 | |a published: Print-Electronic | ||
500 | |a Citation Status MEDLINE | ||
520 | |a Mutations associated with the pol and the S gene can emerge as a consequence of the high replication capacity and proofreading deficiencies of hepatitis B virus during replication. The current study was constructed to evaluate primary, partial, compensatory and the escape mutations in chronic hepatitis B patients in Northern Cyprus. The samples of HBsAg positive treatment naïve 100 patients were involved in this study. HBV pol gene region was sequenced, amplified and HBV pol/S gene mutations were determined. The samples of thirty-two patients were excluded because of their low viral load (HBV < 1000 ıu/ml). Among the sequenced 68 samples, there was a partial mutation (1.5%) and 36.7% displayed a resistance profile to lamivudine, adevofir, and telbivudine. Immune response escape, vaccine escape, HBIg and diagnosis escape mutations were determined in 24%, 10%, 6%, and 4% samples of the patients, respectively. Additionally, there were six different combined mutations. These data underscored that there is no concern for primary mutations in Northern Cyprus, however, we have identified a compensatory mutation (rtV173M) that may have primary mutation characteristics by combining with other mutation patterns. Additionally, HBsAg escape mutants demonstrated that detection of the S gene together with the pol gene mutations might be beneficial and important to monitor the surveillance of S variants | ||
520 | |a Mutations associated with the pol and the S gene can emerge as a consequence of the high replication capacity and proofreading deficiencies of hepatitis B virus during replication. The current study was constructed to evaluate primary, partial, compensatory and the escape mutations in chronic hepatitis B patients in Northern Cyprus. The samples of HBsAg positive treatment naïve 100 patients were involved in this study. HBV pol gene region was sequenced, amplified and HBV pol/S gene mutations were determined. The samples of thirty-two patients were excluded because of their low viral load (HBV < 1000 ıu/ml). Among the sequenced 68 samples, there was a partial mutation (1.5%) and 36.7% displayed a resistance profile to lamivudine, adevofir, and telbivudine. Immune response escape, vaccine escape, HBIg and diagnosis escape mutations were determined in 24%, 10%, 6%, and 4% samples of the patients, respectively. Additionally, there were six different combined mutations. These data underscored that there is no concern for primary mutations in Northern Cyprus, however, we have identified a compensatory mutation (rtV173M) that may have primary mutation characteristics by combining with other mutation patterns. Additionally, HBsAg escape mutants demonstrated that detection of the S gene together with the pol gene mutations might be beneficial and important to monitor the surveillance of S variants | ||
650 | 4 | |a Journal Article | |
650 | 7 | |a Antiviral Agents |2 NLM | |
650 | 7 | |a Gene Products, pol |2 NLM | |
650 | 7 | |a Hepatitis B Surface Antigens |2 NLM | |
650 | 7 | |a Lamivudine |2 NLM | |
650 | 7 | |a 2T8Q726O95 |2 NLM | |
700 | 1 | |a Sayan, Murat |e verfasserin |4 aut | |
700 | 1 | |a Sanlidag, Tamer |e verfasserin |4 aut | |
700 | 1 | |a Suer, Kaya |e verfasserin |4 aut | |
700 | 1 | |a Akcali, Sinem |e verfasserin |4 aut | |
700 | 1 | |a Guvenir, Meryem |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Polish journal of microbiology |d 2004 |g 68(2019), 3 vom: 24. Sept., Seite 317-322 |w (DE-627)NLM150089406 |x 2544-4646 |7 nnns |
773 | 1 | 8 | |g volume:68 |g year:2019 |g number:3 |g day:24 |g month:09 |g pages:317-322 |
856 | 4 | 0 | |u http://dx.doi.org/10.33073/pjm-2019-034 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 68 |j 2019 |e 3 |b 24 |c 09 |h 317-322 |