Analytical techniques for metabolomic studies : a review
Metabolomics is the comprehensive study of small-molecule metabolites. Obtaining a wide coverage of the metabolome is challenging because of the broad range of physicochemical properties of the small molecules. To study the compounds of interest spectroscopic (NMR), spectrometric (MS) and separation techniques (LC, GC, supercritical fluid chromatography, CE) are used. The choice for a given technique is influenced by the sample matrix, the concentration and properties of the metabolites, and the amount of sample. This review discusses the most commonly used analytical techniques for metabolomic studies, including their advantages, drawbacks and some applications.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2019 |
|---|---|
| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:11 |
|---|---|
| Enthalten in: |
Bioanalysis - 11(2019), 24 vom: 25. Dez., Seite 2297-2318 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Segers, Karen [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Journal Article |
|---|
| Anmerkungen: |
Date Completed 21.04.2020 Date Revised 21.04.2020 published: Print Citation Status MEDLINE |
|---|
| doi: |
10.4155/bio-2019-0014 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM304465216 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM304465216 | ||
| 003 | DE-627 | ||
| 005 | 20231225115038.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2019 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.4155/bio-2019-0014 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1014.xml |
| 035 | |a (DE-627)NLM304465216 | ||
| 035 | |a (NLM)31845604 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Segers, Karen |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Analytical techniques for metabolomic studies |b a review |
| 264 | 1 | |c 2019 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 21.04.2020 | ||
| 500 | |a Date Revised 21.04.2020 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Metabolomics is the comprehensive study of small-molecule metabolites. Obtaining a wide coverage of the metabolome is challenging because of the broad range of physicochemical properties of the small molecules. To study the compounds of interest spectroscopic (NMR), spectrometric (MS) and separation techniques (LC, GC, supercritical fluid chromatography, CE) are used. The choice for a given technique is influenced by the sample matrix, the concentration and properties of the metabolites, and the amount of sample. This review discusses the most commonly used analytical techniques for metabolomic studies, including their advantages, drawbacks and some applications | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Review | |
| 650 | 4 | |a multiplatform approaches | |
| 650 | 4 | |a separation techniques | |
| 650 | 4 | |a spectrometric techniques | |
| 650 | 4 | |a spectroscopic techniques | |
| 650 | 4 | |a targeted metabolomics | |
| 650 | 4 | |a untargeted metabolomics | |
| 700 | 1 | |a Declerck, Sven |e verfasserin |4 aut | |
| 700 | 1 | |a Mangelings, Debby |e verfasserin |4 aut | |
| 700 | 1 | |a Heyden, Yvan Vander |e verfasserin |4 aut | |
| 700 | 1 | |a Eeckhaut, Ann Van |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Bioanalysis |d 2009 |g 11(2019), 24 vom: 25. Dez., Seite 2297-2318 |w (DE-627)NLM196969840 |x 1757-6199 |7 nnns |
| 773 | 1 | 8 | |g volume:11 |g year:2019 |g number:24 |g day:25 |g month:12 |g pages:2297-2318 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.4155/bio-2019-0014 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 11 |j 2019 |e 24 |b 25 |c 12 |h 2297-2318 | ||