Endoplasmic reticulum stress is involved in ventilator-induced lung injury in mice via the IRE1α-TRAF2-NF-κB pathway
Copyright © 2019 Elsevier B.V. All rights reserved..
Inflammation plays a criticalrole in the development of ventilator-induced lung injury (VILI). Endoplasmic reticulum (ER) stress is associated with a variety of diseases through the modulation of inflammatory responses. However, little is known about how ER stress is implicated in VILI. In this study, murine mechanical ventilation models were constructed. Total protein and inflammatory cytokines were measured in bronchoalveolar lavage fluid (BALF),and lung tissue injurywasassessedby histology. Our data revealed that mice subjected to high tidal ventilation (TV) for 4 h showed more severe pulmonary edema and inflammation than those of mice with spontaneous breathing and low TV-treatment. In addition, the high TV-treated animals upregulated the ER stress markers GRP78, CHOP, p-IRE1α, TRAF2, and p-NF-κB expression at both the mRNA and protein levels in lung tissue. Administration of thapsigargin exacerbated the histological changes, inflammation and expression of GRP78 and CHOP after high TV, but treatment with ER stress and IRE1α kinase inhibitors attenuated the pathological damage and downregulated the high expression of GRP78, CHOP, p-IRE1α, TRAF2, and p-NF-κB, suggesting that ER stress is involved in VILI though the IRE1α/TRAF2/NF-κB signaling pathway in mice.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:78 |
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| Enthalten in: |
International immunopharmacology - 78(2020) vom: 01. Jan., Seite 106069 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Ye, Liu [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 13.07.2020 Date Revised 04.12.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.intimp.2019.106069 |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM30442756X |
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| 245 | 1 | 0 | |a Endoplasmic reticulum stress is involved in ventilator-induced lung injury in mice via the IRE1α-TRAF2-NF-κB pathway |
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| 520 | |a Copyright © 2019 Elsevier B.V. All rights reserved. | ||
| 520 | |a Inflammation plays a criticalrole in the development of ventilator-induced lung injury (VILI). Endoplasmic reticulum (ER) stress is associated with a variety of diseases through the modulation of inflammatory responses. However, little is known about how ER stress is implicated in VILI. In this study, murine mechanical ventilation models were constructed. Total protein and inflammatory cytokines were measured in bronchoalveolar lavage fluid (BALF),and lung tissue injurywasassessedby histology. Our data revealed that mice subjected to high tidal ventilation (TV) for 4 h showed more severe pulmonary edema and inflammation than those of mice with spontaneous breathing and low TV-treatment. In addition, the high TV-treated animals upregulated the ER stress markers GRP78, CHOP, p-IRE1α, TRAF2, and p-NF-κB expression at both the mRNA and protein levels in lung tissue. Administration of thapsigargin exacerbated the histological changes, inflammation and expression of GRP78 and CHOP after high TV, but treatment with ER stress and IRE1α kinase inhibitors attenuated the pathological damage and downregulated the high expression of GRP78, CHOP, p-IRE1α, TRAF2, and p-NF-κB, suggesting that ER stress is involved in VILI though the IRE1α/TRAF2/NF-κB signaling pathway in mice | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Endoplasmic reticulum stress | |
| 650 | 4 | |a IRE1α/TRAF2/NF-κB signaling pathway | |
| 650 | 4 | |a Inflammation | |
| 650 | 4 | |a Ventilator-induced lung injury | |
| 650 | 7 | |a Cytokines |2 NLM | |
| 650 | 7 | |a Endoplasmic Reticulum Chaperone BiP |2 NLM | |
| 650 | 7 | |a HSPA5 protein, human |2 NLM | |
| 650 | 7 | |a Hspa5 protein, mouse |2 NLM | |
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| 650 | 7 | |a TNF Receptor-Associated Factor 2 |2 NLM | |
| 650 | 7 | |a TRAF2 protein, mouse |2 NLM | |
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| 650 | 7 | |a Endoribonucleases |2 NLM | |
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| 700 | 1 | |a Zeng, Qi |e verfasserin |4 aut | |
| 700 | 1 | |a Dai, Huijun |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Weikang |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Xiaoxia |e verfasserin |4 aut | |
| 700 | 1 | |a Ma, Riliang |e verfasserin |4 aut | |
| 700 | 1 | |a Hong, Xueqi |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Chen |e verfasserin |4 aut | |
| 700 | 1 | |a Pan, Linghui |e verfasserin |4 aut | |
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