Short communication : Effects of a physiologically relevant concentration of aflatoxin B1 with or without sequestering agents on in vitro rumen fermentation of a dairy cow diet
Copyright © 2020 American Dairy Science Association. Published by Elsevier Inc. All rights reserved..
Aflatoxin is a potent carcinogen commonly found in animal feeds that can impair rumen fermentation at high concentrations; however, its effects at physiologically relevant concentrations are unknown. This study examined the effects of aflatoxin B1 (AFB1), with or without bentonite clay (CL) and Saccharomyces cerevisiae fermentation product (SCFP)-based sequestering agents on in vitro rumen fermentation and digestibility of a dairy cow TMR. Corn silage-based TMR (0.5 g, 17.3% crude protein and 1.67 Mcal/kg of net energy for lactation) was incubated in a rumen fluid-buffer inoculum (1:2 ratio; 50 mL) with the following treatments: (1) no additives (control); (2) control + 0.75 µg/L AFB1 (T); (3) T + 80 mg/L sodium bentonite clay (CL; Astra-Ben-20, Prince Agri Products Inc., Quincy, IL); or (4) CL + 14 mg/L SCFP (CL+SCFP; Diamond V, Cedar Rapids, IA). Ruminal fluid was collected 3 h after the morning feeding from 3 cannulated cows fed the same TMR, and rumen fluid from individual cows was used to prepare separate inocula. Each treatment was incubated in duplicate at 39°C for 0, 4, 8, 16, and 24 h in each of 3 runs. Adding T reduced total volatile fatty acid (VFA) concentration after 4 and 8 h and molar proportion of propionate after 4 and 24 h of incubation relative to control. Adding sequestering agents (CL and CL+SCFP) with T did not affect total VFA concentration after 4 or 8 h, but increased total VFA after 16 h and tended to increase molar proportion of propionate after 24 h compared with T. At 24 h, T had lower DM digestibility and higher NH3-N concentration compared with the control. Thus, AFB1, even at very low concentration (0.75 µg/L), had detrimental effects on rumen fermentation and subsequently DM digestibility of the TMR. Adding sequestering agents did not prevent negative effects of T on rumen fermentation within 8 h of incubation; however, sequestering agents were effective after 16 h of incubation.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:103 |
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| Enthalten in: |
Journal of dairy science - 103(2020), 2 vom: 15. Feb., Seite 1559-1565 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Jiang, Y [VerfasserIn] |
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| Links: |
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| Themen: |
1302-78-9 |
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| Anmerkungen: |
Date Completed 11.05.2020 Date Revised 11.05.2020 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.3168/jds.2019-17318 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM303880112 |
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| 100 | 1 | |a Jiang, Y |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Short communication |b Effects of a physiologically relevant concentration of aflatoxin B1 with or without sequestering agents on in vitro rumen fermentation of a dairy cow diet |
| 264 | 1 | |c 2020 | |
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| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
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| 500 | |a Date Completed 11.05.2020 | ||
| 500 | |a Date Revised 11.05.2020 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2020 American Dairy Science Association. Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a Aflatoxin is a potent carcinogen commonly found in animal feeds that can impair rumen fermentation at high concentrations; however, its effects at physiologically relevant concentrations are unknown. This study examined the effects of aflatoxin B1 (AFB1), with or without bentonite clay (CL) and Saccharomyces cerevisiae fermentation product (SCFP)-based sequestering agents on in vitro rumen fermentation and digestibility of a dairy cow TMR. Corn silage-based TMR (0.5 g, 17.3% crude protein and 1.67 Mcal/kg of net energy for lactation) was incubated in a rumen fluid-buffer inoculum (1:2 ratio; 50 mL) with the following treatments: (1) no additives (control); (2) control + 0.75 µg/L AFB1 (T); (3) T + 80 mg/L sodium bentonite clay (CL; Astra-Ben-20, Prince Agri Products Inc., Quincy, IL); or (4) CL + 14 mg/L SCFP (CL+SCFP; Diamond V, Cedar Rapids, IA). Ruminal fluid was collected 3 h after the morning feeding from 3 cannulated cows fed the same TMR, and rumen fluid from individual cows was used to prepare separate inocula. Each treatment was incubated in duplicate at 39°C for 0, 4, 8, 16, and 24 h in each of 3 runs. Adding T reduced total volatile fatty acid (VFA) concentration after 4 and 8 h and molar proportion of propionate after 4 and 24 h of incubation relative to control. Adding sequestering agents (CL and CL+SCFP) with T did not affect total VFA concentration after 4 or 8 h, but increased total VFA after 16 h and tended to increase molar proportion of propionate after 24 h compared with T. At 24 h, T had lower DM digestibility and higher NH3-N concentration compared with the control. Thus, AFB1, even at very low concentration (0.75 µg/L), had detrimental effects on rumen fermentation and subsequently DM digestibility of the TMR. Adding sequestering agents did not prevent negative effects of T on rumen fermentation within 8 h of incubation; however, sequestering agents were effective after 16 h of incubation | ||
| 650 | 4 | |a Clinical Trial, Veterinary | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a aflatoxin | |
| 650 | 4 | |a bentonite clay | |
| 650 | 4 | |a rumen fermentation | |
| 650 | 4 | |a sequestering agent | |
| 650 | 7 | |a Poisons |2 NLM | |
| 650 | 7 | |a Sequestering Agents |2 NLM | |
| 650 | 7 | |a Bentonite |2 NLM | |
| 650 | 7 | |a 1302-78-9 |2 NLM | |
| 650 | 7 | |a Aflatoxin B1 |2 NLM | |
| 650 | 7 | |a 9N2N2Y55MH |2 NLM | |
| 700 | 1 | |a Ogunade, I M |e verfasserin |4 aut | |
| 700 | 1 | |a Arriola, K G |e verfasserin |4 aut | |
| 700 | 1 | |a Pech-Cervantes, A A |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, D H |e verfasserin |4 aut | |
| 700 | 1 | |a Li, X |e verfasserin |4 aut | |
| 700 | 1 | |a Xue, Y L |e verfasserin |4 aut | |
| 700 | 1 | |a Vyas, D |e verfasserin |4 aut | |
| 700 | 1 | |a Adesogan, A T |e verfasserin |4 aut | |
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