A novel PCR-based point-of-care method enables rapid, sensitive and reliable diagnosis of Babesia gibsoni infection in dogs
BACKGROUND: Babesia gibsoni (B. gibsoni) is an intraerythrocytic protozoan parasite of dogs that causes fever and hemolytic illness. A timely diagnosis is essential for the disease management.
RESULTS: Here, we report a QubeMDx PCR system which enables a rapid, sensitive and reliable diagnosis of B. gibsoni near the dog patient. Within 30 min, this diagnostic assay was able to detect as low as 0.002% parasitemia of the dog blood. Using clinical samples, this new assay was validated to demonstrate 100% agreement with real-time PCR.
CONCLUSIONS: This novel diagnostic method provides a reliable point-of-care test to assist in the identification of B. gibsoni.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:15 |
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| Enthalten in: |
BMC veterinary research - 15(2019), 1 vom: 29. Nov., Seite 428 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Liu, I-Li [VerfasserIn] |
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| Links: |
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| Themen: |
Babesia gibsoni |
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| Anmerkungen: |
Date Completed 23.03.2020 Date Revised 23.03.2020 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1186/s12917-019-2181-5 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM303859997 |
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| 100 | 1 | |a Liu, I-Li |e verfasserin |4 aut | |
| 245 | 1 | 2 | |a A novel PCR-based point-of-care method enables rapid, sensitive and reliable diagnosis of Babesia gibsoni infection in dogs |
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| 500 | |a Date Revised 23.03.2020 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a BACKGROUND: Babesia gibsoni (B. gibsoni) is an intraerythrocytic protozoan parasite of dogs that causes fever and hemolytic illness. A timely diagnosis is essential for the disease management | ||
| 520 | |a RESULTS: Here, we report a QubeMDx PCR system which enables a rapid, sensitive and reliable diagnosis of B. gibsoni near the dog patient. Within 30 min, this diagnostic assay was able to detect as low as 0.002% parasitemia of the dog blood. Using clinical samples, this new assay was validated to demonstrate 100% agreement with real-time PCR | ||
| 520 | |a CONCLUSIONS: This novel diagnostic method provides a reliable point-of-care test to assist in the identification of B. gibsoni | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Babesia gibsoni | |
| 650 | 4 | |a Point-of-care | |
| 650 | 4 | |a QubeMDx PCR system | |
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| 700 | 1 | |a Chi, Nai-Yu |e verfasserin |4 aut | |
| 700 | 1 | |a Chang, Chia-Ling |e verfasserin |4 aut | |
| 700 | 1 | |a Hung, Ming-Long |e verfasserin |4 aut | |
| 700 | 1 | |a Chiu, Chun-Ta |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Hui-Wen |e verfasserin |4 aut | |
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