The impact of hemolysis on stability of N-desethyloxybutynin in human plasma
Aim: Stability must be evaluated before quantitation of drugs or metabolites concentrations in biological matrices. We reported a case study where instability of a drug metabolite was mediated by hemolysis. Materials & methods: The instability of both enantiomers of N-desethyloxybutynin was observed in hemolyzed plasma stored at -20°C. The investigations indicated that heme-mediated oxidation converted the metabolite to its N-oxide. Storing samples under lower temperature (-50°C or below) or treatment with the antioxidant ascorbic acid stabilized the metabolite. Conclusion: The evaluation of the stability of some analytes in a hemolyzed sample is crucial as it may negatively impact incurred sample reanalysis or pharmacokinetic profiles on highly hemolyzed samples.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:11 |
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| Enthalten in: |
Bioanalysis - 11(2019), 23 vom: 12. Dez., Seite 2133-2144 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Miao, Zhixin [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 27.04.2020 Date Revised 27.04.2020 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.4155/bio-2019-0160 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM303325127 |
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| 245 | 1 | 4 | |a The impact of hemolysis on stability of N-desethyloxybutynin in human plasma |
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| 500 | |a Date Completed 27.04.2020 | ||
| 500 | |a Date Revised 27.04.2020 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Aim: Stability must be evaluated before quantitation of drugs or metabolites concentrations in biological matrices. We reported a case study where instability of a drug metabolite was mediated by hemolysis. Materials & methods: The instability of both enantiomers of N-desethyloxybutynin was observed in hemolyzed plasma stored at -20°C. The investigations indicated that heme-mediated oxidation converted the metabolite to its N-oxide. Storing samples under lower temperature (-50°C or below) or treatment with the antioxidant ascorbic acid stabilized the metabolite. Conclusion: The evaluation of the stability of some analytes in a hemolyzed sample is crucial as it may negatively impact incurred sample reanalysis or pharmacokinetic profiles on highly hemolyzed samples | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a LC–MS-MS | |
| 650 | 4 | |a N-desethyloxybutynin | |
| 650 | 4 | |a bioanalytical | |
| 650 | 4 | |a chiral separation | |
| 650 | 4 | |a heme-mediated oxidation | |
| 650 | 4 | |a hemolysis | |
| 650 | 4 | |a method development | |
| 650 | 4 | |a oxybutynin | |
| 650 | 4 | |a stability | |
| 650 | 4 | |a validation | |
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| 650 | 7 | |a desethyloxybutynin |2 NLM | |
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| 700 | 1 | |a Tan, Melvin |e verfasserin |4 aut | |
| 700 | 1 | |a Wells, Edward |e verfasserin |4 aut | |
| 700 | 1 | |a Unger, Steve |e verfasserin |4 aut | |
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