Ethanol Extract of Centella asiatica (Gotu Kola) Attenuates Tubular Injury Through Inhibition of Inflammatory Cytokines and Enhancement of Anti-Fibrotic Factor in Mice with 5/6 Subtotal Nephrectomy
© Penerbit Universiti Sains Malaysia, 2019..
BACKGROUND: Chronic kidney disease (CKD) leads to inflammation, fibrosis and destruction of the renal architecture. Centella asiatica (CeA) is an herbaceous plant with anti-inflammatory effects. We aimed to elucidate the effect of CeA on inflammation, fibrosis, vascular remodelling and antifibrotic substances in a 5/6 subtotal nephrectomy (SN) model in mice.
METHODS: Mice were divided into three groups: sham operation (SO, n = 6), 5/6 SN for seven days (SN7, n = 7) and SN7 with oral CeA treatment (SN7-CeA, n = 7). At day 7, mice were euthanised, kidneys were harvested and stained with periodic-acid Schiff (for tubular injury and glomerulosclerosis) and sirius red (for fibrosis and vascular remodeling) staining. mRNA expression of prepro-endothelin-1, nephrin, E-cadherin, bone morphogenic protein-7 (BMP-7), toll-like receptor 4 (TLR4), tumour necrosis factor-α (TNFα) and hepatocyte growth factor (HGF) were quantified using reverse transcriptase-PCR.
RESULTS: SN group demonstrated significant higher interstitial fibrosis, vascular remodeling, tubular injury and glomerulosclerosis (P < 0.01) compared to SO group. Meanwhile, in SN7-CeA demonstrated attenuation of vascular remodeling as shown by significant higher lumen area with lower Wall/Lumen area ratio compared to SN7. RT-PCR analysis showed up-regulation of nephrin, BMP-7 and E-cadherin mRNA expression (P < 0.05) and down-regulation of ppET-1 in SN7-CeA group compared to SN7 group (P < 0.05).
CONCLUSION: CeA may ameliorate renal injury in the SN model in mice.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2019 |
|---|---|
| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:26 |
|---|---|
| Enthalten in: |
The Malaysian journal of medical sciences : MJMS - 26(2019), 5 vom: 01. Sept., Seite 53-63 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Arfian, Nur [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
5/6 subtotal nephrectomy |
|---|
| Anmerkungen: |
Date Revised 19.10.2023 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
|---|
| doi: |
10.21315/mjms2019.26.5.5 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM303313994 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM303313994 | ||
| 003 | DE-627 | ||
| 005 | 20231226200801.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2019 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.21315/mjms2019.26.5.5 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1011.xml |
| 035 | |a (DE-627)NLM303313994 | ||
| 035 | |a (NLM)31728118 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Arfian, Nur |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Ethanol Extract of Centella asiatica (Gotu Kola) Attenuates Tubular Injury Through Inhibition of Inflammatory Cytokines and Enhancement of Anti-Fibrotic Factor in Mice with 5/6 Subtotal Nephrectomy |
| 264 | 1 | |c 2019 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Revised 19.10.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a © Penerbit Universiti Sains Malaysia, 2019. | ||
| 520 | |a BACKGROUND: Chronic kidney disease (CKD) leads to inflammation, fibrosis and destruction of the renal architecture. Centella asiatica (CeA) is an herbaceous plant with anti-inflammatory effects. We aimed to elucidate the effect of CeA on inflammation, fibrosis, vascular remodelling and antifibrotic substances in a 5/6 subtotal nephrectomy (SN) model in mice | ||
| 520 | |a METHODS: Mice were divided into three groups: sham operation (SO, n = 6), 5/6 SN for seven days (SN7, n = 7) and SN7 with oral CeA treatment (SN7-CeA, n = 7). At day 7, mice were euthanised, kidneys were harvested and stained with periodic-acid Schiff (for tubular injury and glomerulosclerosis) and sirius red (for fibrosis and vascular remodeling) staining. mRNA expression of prepro-endothelin-1, nephrin, E-cadherin, bone morphogenic protein-7 (BMP-7), toll-like receptor 4 (TLR4), tumour necrosis factor-α (TNFα) and hepatocyte growth factor (HGF) were quantified using reverse transcriptase-PCR | ||
| 520 | |a RESULTS: SN group demonstrated significant higher interstitial fibrosis, vascular remodeling, tubular injury and glomerulosclerosis (P < 0.01) compared to SO group. Meanwhile, in SN7-CeA demonstrated attenuation of vascular remodeling as shown by significant higher lumen area with lower Wall/Lumen area ratio compared to SN7. RT-PCR analysis showed up-regulation of nephrin, BMP-7 and E-cadherin mRNA expression (P < 0.05) and down-regulation of ppET-1 in SN7-CeA group compared to SN7 group (P < 0.05) | ||
| 520 | |a CONCLUSION: CeA may ameliorate renal injury in the SN model in mice | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a 5/6 subtotal nephrectomy | |
| 650 | 4 | |a Centella asiatica | |
| 650 | 4 | |a anti-fibrotic | |
| 650 | 4 | |a anti-inflammatory | |
| 650 | 4 | |a tubular injury | |
| 700 | 1 | |a Setyaningsih, Wiwit Ananda Wahyu |e verfasserin |4 aut | |
| 700 | 1 | |a Anggorowati, Nungki |e verfasserin |4 aut | |
| 700 | 1 | |a Romi, Muhammad Mansyur |e verfasserin |4 aut | |
| 700 | 1 | |a Sari, Dwi Cahyani Ratna |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t The Malaysian journal of medical sciences : MJMS |d 1999 |g 26(2019), 5 vom: 01. Sept., Seite 53-63 |w (DE-627)NLM217645542 |x 1394-195X |7 nnns |
| 773 | 1 | 8 | |g volume:26 |g year:2019 |g number:5 |g day:01 |g month:09 |g pages:53-63 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.21315/mjms2019.26.5.5 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 26 |j 2019 |e 5 |b 01 |c 09 |h 53-63 | ||