Effects of Spaceflight on Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Structure and Function
Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved..
With extended stays aboard the International Space Station (ISS) becoming commonplace, there is a need to better understand the effects of microgravity on cardiac function. We utilized human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) to study the effects of microgravity on cell-level cardiac function and gene expression. The hiPSC-CMs were cultured aboard the ISS for 5.5 weeks and their gene expression, structure, and functions were compared with ground control hiPSC-CMs. Exposure to microgravity on the ISS caused alterations in hiPSC-CM calcium handling. RNA-sequencing analysis demonstrated that 2,635 genes were differentially expressed among flight, post-flight, and ground control samples, including genes involved in mitochondrial metabolism. This study represents the first use of hiPSC technology to model the effects of spaceflight on human cardiomyocyte structure and function.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:13 |
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| Enthalten in: |
Stem cell reports - 13(2019), 6 vom: 10. Dez., Seite 960-969 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wnorowski, Alexa [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 30.06.2020 Date Revised 30.06.2020 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.stemcr.2019.10.006 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM303122404 |
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| 520 | |a Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a With extended stays aboard the International Space Station (ISS) becoming commonplace, there is a need to better understand the effects of microgravity on cardiac function. We utilized human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) to study the effects of microgravity on cell-level cardiac function and gene expression. The hiPSC-CMs were cultured aboard the ISS for 5.5 weeks and their gene expression, structure, and functions were compared with ground control hiPSC-CMs. Exposure to microgravity on the ISS caused alterations in hiPSC-CM calcium handling. RNA-sequencing analysis demonstrated that 2,635 genes were differentially expressed among flight, post-flight, and ground control samples, including genes involved in mitochondrial metabolism. This study represents the first use of hiPSC technology to model the effects of spaceflight on human cardiomyocyte structure and function | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
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| 650 | 4 | |a Research Support, U.S. Gov't, Non-P.H.S. | |
| 650 | 4 | |a calcium imaging | |
| 650 | 4 | |a cardiology | |
| 650 | 4 | |a cardiomyocytes | |
| 650 | 4 | |a heart | |
| 650 | 4 | |a induced pluripotent stem cells | |
| 650 | 4 | |a metabolism | |
| 650 | 4 | |a microgravity | |
| 650 | 4 | |a modeling | |
| 650 | 4 | |a spaceflight | |
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| 700 | 1 | |a Sharma, Arun |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Haodong |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Haodi |e verfasserin |4 aut | |
| 700 | 1 | |a Shao, Ning-Yi |e verfasserin |4 aut | |
| 700 | 1 | |a Sayed, Nazish |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Chun |e verfasserin |4 aut | |
| 700 | 1 | |a Countryman, Stefanie |e verfasserin |4 aut | |
| 700 | 1 | |a Stodieck, Louis S |e verfasserin |4 aut | |
| 700 | 1 | |a Rubins, Kathleen H |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Sean M |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Peter H U |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Joseph C |e verfasserin |4 aut | |
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