Diagnostic kit for rice blight resistance
Blight-resistant rice lines are the most effective solution for bacterial blight, caused by Xanthomonas oryzae pv. oryzae (Xoo). Key resistance mechanisms involve SWEET genes as susceptibility factors. Bacterial transcription activator-like (TAL) effectors bind to effector-binding elements (EBEs) in SWEET gene promoters and induce SWEET genes. EBE variants that cannot be recognized by TAL effectors abrogate induction, causing resistance. Here we describe a diagnostic kit to enable analysis of bacterial blight in the field and identification of suitable resistant lines. Specifically, we include a SWEET promoter database, RT-PCR primers for detecting SWEET induction, engineered reporter rice lines to visualize SWEET protein accumulation and knock-out rice lines to identify virulence mechanisms in bacterial isolates. We also developed CRISPR-Cas9 genome-edited Kitaake rice to evaluate the efficacy of EBE mutations in resistance, software to predict the optimal resistance gene set for a specific geographic region, and two resistant 'mega' rice lines that will empower farmers to plant lines that are most likely to resist rice blight.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:37 |
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| Enthalten in: |
Nature biotechnology - 37(2019), 11 vom: 28. Nov., Seite 1372-1379 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Eom, Joon-Seob [VerfasserIn] |
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| Links: |
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| Themen: |
Bacterial Proteins |
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| Anmerkungen: |
Date Completed 24.01.2020 Date Revised 20.10.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1038/s41587-019-0268-y |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM302640517 |
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| 520 | |a Blight-resistant rice lines are the most effective solution for bacterial blight, caused by Xanthomonas oryzae pv. oryzae (Xoo). Key resistance mechanisms involve SWEET genes as susceptibility factors. Bacterial transcription activator-like (TAL) effectors bind to effector-binding elements (EBEs) in SWEET gene promoters and induce SWEET genes. EBE variants that cannot be recognized by TAL effectors abrogate induction, causing resistance. Here we describe a diagnostic kit to enable analysis of bacterial blight in the field and identification of suitable resistant lines. Specifically, we include a SWEET promoter database, RT-PCR primers for detecting SWEET induction, engineered reporter rice lines to visualize SWEET protein accumulation and knock-out rice lines to identify virulence mechanisms in bacterial isolates. We also developed CRISPR-Cas9 genome-edited Kitaake rice to evaluate the efficacy of EBE mutations in resistance, software to predict the optimal resistance gene set for a specific geographic region, and two resistant 'mega' rice lines that will empower farmers to plant lines that are most likely to resist rice blight | ||
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| 700 | 1 | |a Yang, Jungil |e verfasserin |4 aut | |
| 700 | 1 | |a Ji, Chonghui |e verfasserin |4 aut | |
| 700 | 1 | |a Thi Luu, Van |e verfasserin |4 aut | |
| 700 | 1 | |a Huguet-Tapia, José C |e verfasserin |4 aut | |
| 700 | 1 | |a Char, Si Nian |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Bo |e verfasserin |4 aut | |
| 700 | 1 | |a Nguyen, Hanna |e verfasserin |4 aut | |
| 700 | 1 | |a Schmidt, Sarah Maria |e verfasserin |4 aut | |
| 700 | 1 | |a Szurek, Boris |e verfasserin |4 aut | |
| 700 | 1 | |a Vera Cruz, Casiana |e verfasserin |4 aut | |
| 700 | 1 | |a White, Frank F |e verfasserin |4 aut | |
| 700 | 1 | |a Oliva, Ricardo |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Bing |e verfasserin |4 aut | |
| 700 | 1 | |a Frommer, Wolf B |e verfasserin |4 aut | |
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