FluChip-8G Insight : HA and NA subtyping of potentially pandemic influenza A viruses in a single assay
© 2019 The Authors. Influenza and Other Respiratory Viruses Published by John Wiley & Sons Ltd..
BACKGROUND: Global influenza surveillance in humans and animals is a critical component of pandemic preparedness. The FluChip-8G Insight assay was developed to subtype both seasonal and potentially pandemic influenza viruses in a single assay with a same day result. FluChip-8G Insight uses whole gene segment RT-PCR-based amplification to provide robustness against genetic drift and subsequent microarray detection with artificial neural network-based data interpretation.
OBJECTIVES: The objective of this study was to verify and validate the performance of the FluChip-8G Insight assay for the detection and positive identification of human and animal origin non-seasonal influenza A specimens.
METHODS: We evaluated the ability of the FluChip-8G Insight technology to type and HA and NA subtype a sample set consisting of 297 results from 180 unique non-seasonal influenza A strains (49 unique subtypes).
RESULTS: FluChip-8G Insight demonstrated a positive percent agreement ≥93% for 5 targeted HA and 5 targeted NA subtypes except for H9 (88%), and negative percent agreement exceeding 95% for all targeted subtypes.
CONCLUSIONS: The FluChip-8G Insight neural network-based algorithm used for virus identification performed well over a data set of 297 naïve sample results, and can be easily updated to improve performance on emerging strains without changing the underlying assay chemistry.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:14 |
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| Enthalten in: |
Influenza and other respiratory viruses - 14(2020), 1 vom: 13. Jan., Seite 55-60 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Toth, Evan [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 28.05.2020 Date Revised 10.01.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1111/irv.12683 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM302153683 |
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| 100 | 1 | |a Toth, Evan |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a FluChip-8G Insight |b HA and NA subtyping of potentially pandemic influenza A viruses in a single assay |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
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| 500 | |a Date Completed 28.05.2020 | ||
| 500 | |a Date Revised 10.01.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2019 The Authors. Influenza and Other Respiratory Viruses Published by John Wiley & Sons Ltd. | ||
| 520 | |a BACKGROUND: Global influenza surveillance in humans and animals is a critical component of pandemic preparedness. The FluChip-8G Insight assay was developed to subtype both seasonal and potentially pandemic influenza viruses in a single assay with a same day result. FluChip-8G Insight uses whole gene segment RT-PCR-based amplification to provide robustness against genetic drift and subsequent microarray detection with artificial neural network-based data interpretation | ||
| 520 | |a OBJECTIVES: The objective of this study was to verify and validate the performance of the FluChip-8G Insight assay for the detection and positive identification of human and animal origin non-seasonal influenza A specimens | ||
| 520 | |a METHODS: We evaluated the ability of the FluChip-8G Insight technology to type and HA and NA subtype a sample set consisting of 297 results from 180 unique non-seasonal influenza A strains (49 unique subtypes) | ||
| 520 | |a RESULTS: FluChip-8G Insight demonstrated a positive percent agreement ≥93% for 5 targeted HA and 5 targeted NA subtypes except for H9 (88%), and negative percent agreement exceeding 95% for all targeted subtypes | ||
| 520 | |a CONCLUSIONS: The FluChip-8G Insight neural network-based algorithm used for virus identification performed well over a data set of 297 naïve sample results, and can be easily updated to improve performance on emerging strains without changing the underlying assay chemistry | ||
| 650 | 4 | |a Evaluation Study | |
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a Research Support, U.S. Gov't, P.H.S. | |
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| 700 | 1 | |a Taylor, Amber W |e verfasserin |4 aut | |
| 700 | 1 | |a Stoughton, Robert S |e verfasserin |4 aut | |
| 700 | 1 | |a Blair, Rebecca H |e verfasserin |4 aut | |
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| 700 | 1 | |a Fessler, Ryan |e verfasserin |4 aut | |
| 700 | 1 | |a Smith, Catherine B |e verfasserin |4 aut | |
| 700 | 1 | |a Talbot, Sarah |e verfasserin |4 aut | |
| 700 | 1 | |a Rowlen, Kathy |e verfasserin |4 aut | |
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