Contribution of endothelial cell and macrophage activation in the alterations induced by the venom of Micrurus tener tener in C57BL/6 mice
Copyright © 2019. Published by Elsevier Ltd..
An acute inflammatory response, cellular infiltrates, anemia, hemorrhage and endogenous fibrinolysis activation were previously described in C57BL/6 mice injected with M. tener tener venom (Mtt). As the endothelium and innate immunity may participate in these disturbances and due to our poor understanding of the alterations produced by these venoms when the neurotoxic component is not predominant, we evaluated the effects in an in vitro model. At 24 h, the release of pro-inflammatory mediators was detected in peritoneal macrophages. At different times, the release of pro-inflammatory (TNF-α, IL-6, NO and E-Selectin), pro-coagulant (vWF and TF) and pro-fibrinolytic (uPA) mediators were seen in liver sinusoidal endothelial cells (LSECs). These results suggest that Mtt venom activates macrophages and endothelium, thus inducing the release of mediators, such as TNF-α, that orchestrate the acute inflammatory response and the later infiltration of mononuclear cells into liver in C57BL/6 mice. In addition, endothelium activation promotes TF expression, which may in turn modulate the inflammatory and hemostatic response. These findings suggest crosstalk between inflammation and hemostasis in the alterations observed in Micrurus envenomation, where the neurotoxic manifestations do not predominate.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:116 |
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| Enthalten in: |
Molecular immunology - 116(2019) vom: 30. Dez., Seite 45-55 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Salazar, Emelyn [VerfasserIn] |
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| Links: |
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| Themen: |
Elapid Venoms |
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| Anmerkungen: |
Date Completed 22.01.2020 Date Revised 22.01.2020 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.molimm.2019.09.009 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM302075321 |
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| 245 | 1 | 0 | |a Contribution of endothelial cell and macrophage activation in the alterations induced by the venom of Micrurus tener tener in C57BL/6 mice |
| 264 | 1 | |c 2019 | |
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| 500 | |a Date Revised 22.01.2020 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2019. Published by Elsevier Ltd. | ||
| 520 | |a An acute inflammatory response, cellular infiltrates, anemia, hemorrhage and endogenous fibrinolysis activation were previously described in C57BL/6 mice injected with M. tener tener venom (Mtt). As the endothelium and innate immunity may participate in these disturbances and due to our poor understanding of the alterations produced by these venoms when the neurotoxic component is not predominant, we evaluated the effects in an in vitro model. At 24 h, the release of pro-inflammatory mediators was detected in peritoneal macrophages. At different times, the release of pro-inflammatory (TNF-α, IL-6, NO and E-Selectin), pro-coagulant (vWF and TF) and pro-fibrinolytic (uPA) mediators were seen in liver sinusoidal endothelial cells (LSECs). These results suggest that Mtt venom activates macrophages and endothelium, thus inducing the release of mediators, such as TNF-α, that orchestrate the acute inflammatory response and the later infiltration of mononuclear cells into liver in C57BL/6 mice. In addition, endothelium activation promotes TF expression, which may in turn modulate the inflammatory and hemostatic response. These findings suggest crosstalk between inflammation and hemostasis in the alterations observed in Micrurus envenomation, where the neurotoxic manifestations do not predominate | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Endothelium | |
| 650 | 4 | |a Hemostasis | |
| 650 | 4 | |a Inflammation | |
| 650 | 4 | |a Macrophages | |
| 650 | 4 | |a Micrurus tener tener | |
| 650 | 4 | |a Snake venom | |
| 650 | 7 | |a Elapid Venoms |2 NLM | |
| 700 | 1 | |a Salazar, Ana María |e verfasserin |4 aut | |
| 700 | 1 | |a Taylor, Peter |e verfasserin |4 aut | |
| 700 | 1 | |a Urdanibia, Izaskun |e verfasserin |4 aut | |
| 700 | 1 | |a Pérez, Karin |e verfasserin |4 aut | |
| 700 | 1 | |a Rodríguez-Acosta, Alexis |e verfasserin |4 aut | |
| 700 | 1 | |a Sánchez, Elda E |e verfasserin |4 aut | |
| 700 | 1 | |a Guerrero, Belsy |e verfasserin |4 aut | |
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