A new chemical approach for proximity labelling of chromatin-associated RNAs and proteins with visible light irradiation
A new nucleus-localized singlet oxygen generator was designed and synthesized. Its capability to label nucleus-localized biomolecules through spatially-restricted oxidation under visible light was validated using fluorescence confocal microscopy. Western blot and RT-qPCR were performed to verify the desirable spatial resolution through enriching chromatin-associated RNAs and proteins.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:55 |
|---|---|
| Enthalten in: |
Chemical communications (Cambridge, England) - 55(2019), 82 vom: 10. Okt., Seite 12340-12343 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Li, Lan [VerfasserIn] |
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| Links: |
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| Themen: |
17778-80-2 |
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| Anmerkungen: |
Date Completed 31.10.2019 Date Revised 13.11.2019 published: Print Citation Status MEDLINE |
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| doi: |
10.1039/c9cc06251c |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM301646163 |
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| 245 | 1 | 2 | |a A new chemical approach for proximity labelling of chromatin-associated RNAs and proteins with visible light irradiation |
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| 520 | |a A new nucleus-localized singlet oxygen generator was designed and synthesized. Its capability to label nucleus-localized biomolecules through spatially-restricted oxidation under visible light was validated using fluorescence confocal microscopy. Western blot and RT-qPCR were performed to verify the desirable spatial resolution through enriching chromatin-associated RNAs and proteins | ||
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| 700 | 1 | |a Luo, Hao |e verfasserin |4 aut | |
| 700 | 1 | |a Tam, K Ming |e verfasserin |4 aut | |
| 700 | 1 | |a Tse, Edmund C M |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Ying |e verfasserin |4 aut | |
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