Probing the degradation mechanism of forsythiaside A and simultaneous determination of three forsythiasides in Forsythia preparations by a single marker
© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim..
Forsythiaside A is the major component of Forsythia suspensa. This study investigated the degradation mechanism of forsythiaside A. Eight degraded components including forsythiaside I, forsythiaside H, forsythiaside E, caffeic acid, suspensaside A, β-hydroxy forsythiaside I, β-hydroxy forsythiaside H, and β-hydroxy forsythiaside A were identified by using ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry. Then, the quantitative analysis of multi-components by a single-marker was performed with ultra-high performance liquid chromatography to simultaneously determine forsythiaside A, forsythiaside H, and forsythiaside I in Forsythia suspensa preparations. The result showed good linear relationships within 2.871-287.1, 0.231-23.1, and 0.983-98.3 μg/mL (r > 0.9998), with average recoveries of 97.7, 95.7, and 95.8% and relative standard deviations of 1.4, 2.4, and 1.8%, respectively. Using forsythiaside A as an internal reference, the relative retention values of forsythiaside H and forsythiaside I to forsythiaside A were calculated to be 0.89 and 0.61, respectively, and the relative correction factors were 0.816 and 0.799, respectively. The method for quantitative analysis of multi-components by a single-marker was applied to evaluate the overall quality of forsythia preparations. There was no significant difference in the measurement results of the method developed and the method of external standard.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:42 |
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| Enthalten in: |
Journal of separation science - 42(2019), 23 vom: 02. Dez., Seite 3503-3511 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Qi, Minghui [VerfasserIn] |
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| Links: |
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| Themen: |
Degradation pathway |
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| Anmerkungen: |
Date Completed 10.04.2020 Date Revised 10.04.2020 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1002/jssc.201900521 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
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| 520 | |a Forsythiaside A is the major component of Forsythia suspensa. This study investigated the degradation mechanism of forsythiaside A. Eight degraded components including forsythiaside I, forsythiaside H, forsythiaside E, caffeic acid, suspensaside A, β-hydroxy forsythiaside I, β-hydroxy forsythiaside H, and β-hydroxy forsythiaside A were identified by using ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry. Then, the quantitative analysis of multi-components by a single-marker was performed with ultra-high performance liquid chromatography to simultaneously determine forsythiaside A, forsythiaside H, and forsythiaside I in Forsythia suspensa preparations. The result showed good linear relationships within 2.871-287.1, 0.231-23.1, and 0.983-98.3 μg/mL (r > 0.9998), with average recoveries of 97.7, 95.7, and 95.8% and relative standard deviations of 1.4, 2.4, and 1.8%, respectively. Using forsythiaside A as an internal reference, the relative retention values of forsythiaside H and forsythiaside I to forsythiaside A were calculated to be 0.89 and 0.61, respectively, and the relative correction factors were 0.816 and 0.799, respectively. The method for quantitative analysis of multi-components by a single-marker was applied to evaluate the overall quality of forsythia preparations. There was no significant difference in the measurement results of the method developed and the method of external standard | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a forsythiasides | |
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| 650 | 4 | |a traditional Chinese medicine | |
| 650 | 4 | |a ultra high performance liquid chromatography | |
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| 700 | 1 | |a Zhang, Min |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Hongyang |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Yuerong |e verfasserin |4 aut | |
| 700 | 1 | |a Hu, Ping |e verfasserin |4 aut | |
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