Generation of Large Fragment Knock-In Mouse Models by Microinjecting into 2-Cell Stage Embryos
Large fragment knock-in mouse models such as reporters and conditional mutant mice are important models for biological research. Here we describe 2-cell (2C)-homologous recombination (HR)-CRISPR, a highly efficient method to generate large fragment knock-in mouse models by CRISPR-based genome engineering. Using this method, knock-in founders can be generated routinely in a time frame of about two months with high germline transmission efficiency. 2C-HR-CRISPR will significantly promote the advancement of basic and translational research using genetic mouse models.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2066 |
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| Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 2066(2020) vom: 12., Seite 89-100 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Gu, Bin [VerfasserIn] |
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| Links: |
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| Themen: |
2-Cell stage |
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| Anmerkungen: |
Date Completed 14.12.2020 Date Revised 14.12.2020 published: Print Citation Status MEDLINE |
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| doi: |
10.1007/978-1-4939-9837-1_7 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM301208247 |
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| 520 | |a Large fragment knock-in mouse models such as reporters and conditional mutant mice are important models for biological research. Here we describe 2-cell (2C)-homologous recombination (HR)-CRISPR, a highly efficient method to generate large fragment knock-in mouse models by CRISPR-based genome engineering. Using this method, knock-in founders can be generated routinely in a time frame of about two months with high germline transmission efficiency. 2C-HR-CRISPR will significantly promote the advancement of basic and translational research using genetic mouse models | ||
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