Standardizing the determination and interpretation of Pcrit in fishes
© 2019. Published by The Company of Biologists Ltd..
The critical oxygen tension (Pcrit) for fishes is the oxygen level below which the rate of oxygen consumption (ṀO2 ) becomes dependent upon ambient oxygen partial pressure (PO2 ). We compare multiple curve-fitting approaches to estimate Pcrit of the Gulf killifish, Fundulus grandis, during closed and intermittent-flow respirometry. Fitting two line segments of ṀO2 versus PO2 produced high and variable estimates of Pcrit, as did nonlinear regression using a hyperbolic (Michaelis-Menten) function. Using nonlinear regression fit to an exponential (modified Weibull) function, or linear regression of ṀO2 versus PO2 at low PO2 , and determining Pcrit as the PO2 when ṀO2 equals standard metabolic rate (SMR) yielded values that were consistent across fish and among experimental trials. The magnitude of the difference in Pcrit determined by alternative calculation methods exceeded the differences determined in closed and intermittent-flow respirometry, highlighting the need to standardize analytical as well as experimental approaches in determining Pcrit.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:222 |
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| Enthalten in: |
The Journal of experimental biology - 222(2019), Pt 18 vom: 26. Sept. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Reemeyer, Jessica E [VerfasserIn] |
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| Links: |
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| Themen: |
Aerobic metabolism |
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| Anmerkungen: |
Date Completed 05.08.2020 Date Revised 05.08.2020 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1242/jeb.210633 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM301199752 |
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| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2019. Published by The Company of Biologists Ltd. | ||
| 520 | |a The critical oxygen tension (Pcrit) for fishes is the oxygen level below which the rate of oxygen consumption (ṀO2 ) becomes dependent upon ambient oxygen partial pressure (PO2 ). We compare multiple curve-fitting approaches to estimate Pcrit of the Gulf killifish, Fundulus grandis, during closed and intermittent-flow respirometry. Fitting two line segments of ṀO2 versus PO2 produced high and variable estimates of Pcrit, as did nonlinear regression using a hyperbolic (Michaelis-Menten) function. Using nonlinear regression fit to an exponential (modified Weibull) function, or linear regression of ṀO2 versus PO2 at low PO2 , and determining Pcrit as the PO2 when ṀO2 equals standard metabolic rate (SMR) yielded values that were consistent across fish and among experimental trials. The magnitude of the difference in Pcrit determined by alternative calculation methods exceeded the differences determined in closed and intermittent-flow respirometry, highlighting the need to standardize analytical as well as experimental approaches in determining Pcrit | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a Aerobic metabolism | |
| 650 | 4 | |a Critical oxygen tension | |
| 650 | 4 | |a Fundulus grandis | |
| 650 | 4 | |a Hypoxia | |
| 650 | 4 | |a Killifish | |
| 650 | 4 | |a Oxygen consumption | |
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| 700 | 1 | |a Rees, Bernard B |e verfasserin |4 aut | |
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