Single glomerular proteomics : A novel tool for translational glomerular cell biology
© 2019 Elsevier Inc. All rights reserved..
The glomerulus harbors the renal filtration barrier and needs to be precisely maintained. In this chapter, the concept of single glomerular proteomics is described. Single glomerular proteomics has recently been enabled by advances in glomerular isolation, ultrasensitive peptide sample preparation and mass spectrometry based technology and acquisition strategies. It generates protein content information on a single glomerulus that can be overlaid with morphological and other multi-layered omics analyses. The novel method consists of four essential steps: preparation of single glomeruli-by microdissection, glomerular preparation, or laser microdissection-followed by proteomic sample preparation, mass spectrometry analysis and bioinformatics analysis. It enables for the first time the generation of sub-biopsy level proteomics data. In perspective, comprehensive data from individual glomeruli could be used in order to pinpoint novel druggable targets in animal models of kidney disease or in patients with proteinuria and glomerular disease.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2019 |
|---|---|
| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:154 |
|---|---|
| Enthalten in: |
Methods in cell biology - 154(2019) vom: 20., Seite 1-14 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Rinschen, Markus M [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Diabetic nephropathy |
|---|
| Anmerkungen: |
Date Completed 30.04.2020 Date Revised 04.12.2021 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/bs.mcb.2019.03.010 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM301027412 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM301027412 | ||
| 003 | DE-627 | ||
| 005 | 20231225103757.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2019 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/bs.mcb.2019.03.010 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1003.xml |
| 035 | |a (DE-627)NLM301027412 | ||
| 035 | |a (NLM)31493812 | ||
| 035 | |a (PII)S0091-679X(19)30032-9 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Rinschen, Markus M |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Single glomerular proteomics |b A novel tool for translational glomerular cell biology |
| 264 | 1 | |c 2019 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 30.04.2020 | ||
| 500 | |a Date Revised 04.12.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2019 Elsevier Inc. All rights reserved. | ||
| 520 | |a The glomerulus harbors the renal filtration barrier and needs to be precisely maintained. In this chapter, the concept of single glomerular proteomics is described. Single glomerular proteomics has recently been enabled by advances in glomerular isolation, ultrasensitive peptide sample preparation and mass spectrometry based technology and acquisition strategies. It generates protein content information on a single glomerulus that can be overlaid with morphological and other multi-layered omics analyses. The novel method consists of four essential steps: preparation of single glomeruli-by microdissection, glomerular preparation, or laser microdissection-followed by proteomic sample preparation, mass spectrometry analysis and bioinformatics analysis. It enables for the first time the generation of sub-biopsy level proteomics data. In perspective, comprehensive data from individual glomeruli could be used in order to pinpoint novel druggable targets in animal models of kidney disease or in patients with proteinuria and glomerular disease | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Diabetic nephropathy | |
| 650 | 4 | |a Endothelium | |
| 650 | 4 | |a Focal segmental glomerular sclerosis | |
| 650 | 4 | |a Hypertensive kidney disease | |
| 650 | 4 | |a Mesangial cells | |
| 650 | 4 | |a Podocyte | |
| 650 | 4 | |a SP3 | |
| 650 | 4 | |a Single nephron | |
| 650 | 4 | |a Ultrasensitive proteomics | |
| 650 | 7 | |a Peptides |2 NLM | |
| 650 | 7 | |a Proteome |2 NLM | |
| 773 | 0 | 8 | |i Enthalten in |t Methods in cell biology |d 1973 |g 154(2019) vom: 20., Seite 1-14 |w (DE-627)NLM000007870 |x 0091-679X |7 nnns |
| 773 | 1 | 8 | |g volume:154 |g year:2019 |g day:20 |g pages:1-14 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/bs.mcb.2019.03.010 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 154 |j 2019 |b 20 |h 1-14 | ||