Identification of a novel antiviral micro-RNA targeting the NS1 protein of the H1N1 pandemic human influenza virus and a corresponding viral escape mutation
Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved..
The influenza A virus (IAV) NS1 protein is one of the major regulators of pathogenicity, being able to suppress innate immune response and host protein synthesis. In this study we identified the human micro RNA hsa-miR-1307-3p as a novel potent suppressor of NS1 expression and influenza virus replication. Transcriptomic analysis indicates that hsa-miR-1307-3p also negatively regulates apoptosis and promotes cell proliferation. In addition, we identified a novel mutation in the NS1 gene of A(H1N1)pdm09 strains circulating in Italy in the 2010-11 season, which enabled the virus to escape the hsa-miR-1307-3p inhibition, conferring replicative advantage to the virus in human cells. To the best of our knowledge, this is the first validation of suppression of IAV H1N1 NS1 by a human micro RNA and the first example of an escape mutation from micro RNA-mediated antiviral response for the A(H1N1)pdm09 virus.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2019 |
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Erschienen: |
2019 |
Enthalten in: |
Zur Gesamtaufnahme - volume:171 |
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Enthalten in: |
Antiviral research - 171(2019) vom: 01. Nov., Seite 104593 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Bavagnoli, Laura [VerfasserIn] |
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Links: |
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Anmerkungen: |
Date Completed 08.07.2020 Date Revised 08.07.2020 published: Print-Electronic Citation Status MEDLINE |
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doi: |
10.1016/j.antiviral.2019.104593 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM300793510 |
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245 | 1 | 0 | |a Identification of a novel antiviral micro-RNA targeting the NS1 protein of the H1N1 pandemic human influenza virus and a corresponding viral escape mutation |
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500 | |a published: Print-Electronic | ||
500 | |a Citation Status MEDLINE | ||
520 | |a Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved. | ||
520 | |a The influenza A virus (IAV) NS1 protein is one of the major regulators of pathogenicity, being able to suppress innate immune response and host protein synthesis. In this study we identified the human micro RNA hsa-miR-1307-3p as a novel potent suppressor of NS1 expression and influenza virus replication. Transcriptomic analysis indicates that hsa-miR-1307-3p also negatively regulates apoptosis and promotes cell proliferation. In addition, we identified a novel mutation in the NS1 gene of A(H1N1)pdm09 strains circulating in Italy in the 2010-11 season, which enabled the virus to escape the hsa-miR-1307-3p inhibition, conferring replicative advantage to the virus in human cells. To the best of our knowledge, this is the first validation of suppression of IAV H1N1 NS1 by a human micro RNA and the first example of an escape mutation from micro RNA-mediated antiviral response for the A(H1N1)pdm09 virus | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, Non-U.S. Gov't | |
650 | 4 | |a Antiviral micro-RNA | |
650 | 4 | |a Escape mutation | |
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700 | 1 | |a Campanini, Giulia |e verfasserin |4 aut | |
700 | 1 | |a Forte, Maurizio |e verfasserin |4 aut | |
700 | 1 | |a Ceccotti, Giorgia |e verfasserin |4 aut | |
700 | 1 | |a Percivalle, Elena |e verfasserin |4 aut | |
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700 | 1 | |a Lisa, Antonella |e verfasserin |4 aut | |
700 | 1 | |a Baldanti, Fausto |e verfasserin |4 aut | |
700 | 1 | |a Maga, Giovanni |e verfasserin |4 aut | |
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