Novel gain-of-function mutation of TRPV4 associated with accelerated chondrogenic differentiation of dental pulp stem cells derived from a patient with metatropic dysplasia

Metatropic dysplasia is a congenital skeletal dysplasia characterized by severe platyspondyly, dumbbell-like deformity of long tubular bones, and progressive kyphoscoliosis with growth. It is caused by mutations in the gene TRPV4, encoding the transient receptor potential vanilloid 4, which acts as a calcium channel. Many heterozygous single base mutations of this gene have been associated with the disorder, showing autosomal dominant inheritance. Although abnormal endochondral ossification has been observed by histological examination of bone in a patient with lethal metatropic dysplasia, the etiology of the disorder remains largely unresolved. As dental pulp stem cells (DPSCs) are mesenchymal stem cells that differentiate into bone lineage cells, DPSCs derived from patients with congenital skeletal dysplasia might be useful as a disease-specific cellular model for etiological investigation. The purpose of this study was to clarify the pathological association between TRPV4 mutation and chondrocyte differentiation by analyzing DPSCs from a patient with non-lethal metatropic dysplasia. We identified a novel heterozygous single base mutation, c.1855C>T in TRPV4. This was predicted to be a missense mutation, p.L619F, in putative transmembrane segment 5. The mutation was repaired by CRISPR/Cas9 system to obtain isogenic control DPSCs for further analysis. The expression of stem cell markers and fibroblast-like morphology were comparable between patient-derived mutant and control DPSCs, although expression of TRPV4 was lower in mutant DPSCs than control DPSCs. Despite the lower TRPV4 expression in mutant DPSCs, the intracellular Ca2+ level was comparable at the basal level between mutant and control DPSCs, while its level was markedly higher following stimulation with 4α-phorbol 12,13-didecanoate (4αPDD), a specific agonist for TRPV4, in mutant DPSCs than in control DPSCs. In the presence of 4αPDD, we observed accelerated early chondrocyte differentiation and upregulated mRNA expression of SRY-box 9 (SOX9) in mutant DPSCs. Our findings suggested that the novel missense mutation c.1855C>T of TRPV4 was a gain-of-function mutation leading to enhanced intracellular Ca2+ level, which was associated with accelerated chondrocyte differentiation and SOX9 upregulation. Our results also suggest that patient-derived DPSCs can be a useful disease-specific cellular model for elucidating the pathological mechanism of metatropic dysplasia.

Medienart:	E-Artikel

Erscheinungsjahr:	2019
Erschienen:	2019

Enthalten in:	Zur Gesamtaufnahme - volume:19
Enthalten in:	Biochemistry and biophysics reports - 19(2019) vom: 27. Sept., Seite 100648

Sprache:	Englisch

Beteiligte Personen:	Nonaka, Kentaro [VerfasserIn] Han, Xu [VerfasserIn] Kato, Hiroki [VerfasserIn] Sato, Hiroshi [VerfasserIn] Yamaza, Haruyoshi [VerfasserIn] Hirofuji, Yuta [VerfasserIn] Masuda, Keiji [VerfasserIn]

Links:	Volltext

Themen:	4αPDD, 4α-phorbol 12,13-didecanoate CC, chondrocyte Chondrocyte differentiation DPSC, dental pulp stem cell Dental pulp stem cell Journal Article MD, metatropic dysplasia Metatropic dysplasia SOX9, SRY-box 9 TRPV4, transient receptor potential vanilloid 4 Transient receptor potential vanilloid 4

Anmerkungen:	Date Revised 01.10.2020 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE

doi:	10.1016/j.bbrep.2019.100648

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PPN (Katalog-ID):	NLM300728336