7,8-Dihydroxyflavone activates Nrf2/HO-1 signaling pathways and protects against osteoarthritis
The aim of the present study was to investigate the effect of 7,8-dihydroxyflavone (7,8-DHF) against osteoarthritis (OA) and examine its regulatory role in the nuclear factor (erythroid-derived 2)-like 2 (Nrf2) signaling pathway in chondrocytes. Primary mouse chondrocytes were treated with 7,8-DHF to examine the expression of Nrf2 and downstream heme oxygenase 1 (HO-1). The surgical destabilization of the medial meniscus model was used to assess the effectiveness of 7,8-DHF in protecting the cartilage from damage, with knee cartilage harvested from mice for histological analysis. The results revealed that 7,8-DHF activated the Nrf2 signaling pathway in primary chondrocytes. Cartilage degradation in the 7,8-DHF-treated group was reduced significantly compared with that in the vehicle-treated group, according to histological evaluation. The gene expression of matrix metalloproteinase (MMP)1, MMP3, MMP13, interleukin (IL)-1β, IL-6 and tumor necrosis factor-α were reduced in the cartilage of OA mice following 7,8-DHF treatment. Genetic and protein analyses indicated that the expression levels of HO-1 were upregulated in the cartilage of the knee with OA, and 7,8-DHF treatment further promoted the induction of HO-1. These results suggest that 7,8-DHF may serve as a potential therapeutic agent in OA.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:18 |
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| Enthalten in: |
Experimental and therapeutic medicine - 18(2019), 3 vom: 13. Sept., Seite 1677-1684 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Cai, Dawei [VerfasserIn] |
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| Links: |
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| Themen: |
7,8-dihydroxyflavone |
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| Anmerkungen: |
Date Revised 30.09.2020 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
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| doi: |
10.3892/etm.2019.7745 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM300205570 |
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| 520 | |a The aim of the present study was to investigate the effect of 7,8-dihydroxyflavone (7,8-DHF) against osteoarthritis (OA) and examine its regulatory role in the nuclear factor (erythroid-derived 2)-like 2 (Nrf2) signaling pathway in chondrocytes. Primary mouse chondrocytes were treated with 7,8-DHF to examine the expression of Nrf2 and downstream heme oxygenase 1 (HO-1). The surgical destabilization of the medial meniscus model was used to assess the effectiveness of 7,8-DHF in protecting the cartilage from damage, with knee cartilage harvested from mice for histological analysis. The results revealed that 7,8-DHF activated the Nrf2 signaling pathway in primary chondrocytes. Cartilage degradation in the 7,8-DHF-treated group was reduced significantly compared with that in the vehicle-treated group, according to histological evaluation. The gene expression of matrix metalloproteinase (MMP)1, MMP3, MMP13, interleukin (IL)-1β, IL-6 and tumor necrosis factor-α were reduced in the cartilage of OA mice following 7,8-DHF treatment. Genetic and protein analyses indicated that the expression levels of HO-1 were upregulated in the cartilage of the knee with OA, and 7,8-DHF treatment further promoted the induction of HO-1. These results suggest that 7,8-DHF may serve as a potential therapeutic agent in OA | ||
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| 650 | 4 | |a 7,8-dihydroxyflavone | |
| 650 | 4 | |a chondrocytes | |
| 650 | 4 | |a heme oxygenase-1 | |
| 650 | 4 | |a nuclear factor (erythroid-derived 2)-like 2 | |
| 650 | 4 | |a osteoarthritis | |
| 700 | 1 | |a Feng, Wan |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Jun |e verfasserin |4 aut | |
| 700 | 1 | |a Jiang, Longhai |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Sichun |e verfasserin |4 aut | |
| 700 | 1 | |a Yuan, Tangbo |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Chen |e verfasserin |4 aut | |
| 700 | 1 | |a Xie, Hao |e verfasserin |4 aut | |
| 700 | 1 | |a Geng, Dawei |e verfasserin |4 aut | |
| 700 | 1 | |a Qin, Jian |e verfasserin |4 aut | |
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