Ultrastructural Analyses of Microglial Interactions with Synapses
Immunohistochemical electron microscopy (EM) allows the identification of microglial cell bodies and processes, which are otherwise difficult to recognize based on their ultrastructural features. The technique has been essential in defining, at high spatial resolution, microglial interactions with neurons and synapses, thus providing, among other discoveries, important insights into their roles in synaptic pruning and stripping. In this protocol, we describe the preparation of mouse brain tissue for EM, the immunocytochemical staining against ionized calcium binding adaptor molecule 1, the imaging of microglial cell bodies and processes, and the analysis of microglial relationships with the synaptic neuropil.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2019 |
|---|---|
| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2034 |
|---|---|
| Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 2034(2019) vom: 07., Seite 83-95 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Tremblay, Marie-Ève [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Brain |
|---|
| Anmerkungen: |
Date Completed 01.04.2020 Date Revised 09.11.2021 published: Print Citation Status MEDLINE |
|---|
| doi: |
10.1007/978-1-4939-9658-2_7 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM300033826 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM300033826 | ||
| 003 | DE-627 | ||
| 005 | 20231225101638.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2019 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/978-1-4939-9658-2_7 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1000.xml |
| 035 | |a (DE-627)NLM300033826 | ||
| 035 | |a (NLM)31392679 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Tremblay, Marie-Ève |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Ultrastructural Analyses of Microglial Interactions with Synapses |
| 264 | 1 | |c 2019 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 01.04.2020 | ||
| 500 | |a Date Revised 09.11.2021 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Immunohistochemical electron microscopy (EM) allows the identification of microglial cell bodies and processes, which are otherwise difficult to recognize based on their ultrastructural features. The technique has been essential in defining, at high spatial resolution, microglial interactions with neurons and synapses, thus providing, among other discoveries, important insights into their roles in synaptic pruning and stripping. In this protocol, we describe the preparation of mouse brain tissue for EM, the immunocytochemical staining against ionized calcium binding adaptor molecule 1, the imaging of microglial cell bodies and processes, and the analysis of microglial relationships with the synaptic neuropil | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Brain | |
| 650 | 4 | |a Electron Microscopy | |
| 650 | 4 | |a Imaging | |
| 650 | 4 | |a Immunohistochemistry | |
| 650 | 4 | |a Microglia | |
| 650 | 4 | |a Mouse | |
| 650 | 4 | |a Synapses | |
| 650 | 4 | |a Ultrastructural Analysis | |
| 700 | 1 | |a Majewska, Ania K |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Methods in molecular biology (Clifton, N.J.) |d 1984 |g 2034(2019) vom: 07., Seite 83-95 |w (DE-627)NLM074849794 |x 1940-6029 |7 nnns |
| 773 | 1 | 8 | |g volume:2034 |g year:2019 |g day:07 |g pages:83-95 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1007/978-1-4939-9658-2_7 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 2034 |j 2019 |b 07 |h 83-95 | ||