Dual-modality imaging of atherosclerotic plaques using ultrasmall superparamagnetic iron oxide labeled with rhodamine
Aim: The diagnosis of vulnerable atherosclerotic plaques remains challenging. This study labeled ultrasmall superparamagnetic iron oxide with rhodamine (USPIO-R) and evaluated USPIO-R for imaging atherosclerotic plaques. Methods: Apolipoprotein E-deficient mice were fed a high-fat diet and underwent MRI before and after an intravenous injection of USPIO-R. Subsequently, an aortic specimen from the mice was removed and sliced for fluorescence imaging and Prussian blue and immunofluorescent staining. Results: T2 signal loss appeared and persisted in the aortic plaque postinjection, and spontaneous fluorescence from the plaque was observed. The accumulated mechanism of USPIO-R by plaque was the macrophage internalization by Prussian blue and immunofluorescence. Conclusion: USPIO-R is a promising dual-modality probe for diagnosing and monitoring vulnerable atherosclerotic plaques.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:14 |
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| Enthalten in: |
Nanomedicine (London, England) - 14(2019), 15 vom: 15. Aug., Seite 1935-1944 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Li, Yi [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 18.05.2020 Date Revised 18.05.2020 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.2217/nnm-2019-0062 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM299673715 |
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| 245 | 1 | 0 | |a Dual-modality imaging of atherosclerotic plaques using ultrasmall superparamagnetic iron oxide labeled with rhodamine |
| 264 | 1 | |c 2019 | |
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| 500 | |a Date Completed 18.05.2020 | ||
| 500 | |a Date Revised 18.05.2020 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Aim: The diagnosis of vulnerable atherosclerotic plaques remains challenging. This study labeled ultrasmall superparamagnetic iron oxide with rhodamine (USPIO-R) and evaluated USPIO-R for imaging atherosclerotic plaques. Methods: Apolipoprotein E-deficient mice were fed a high-fat diet and underwent MRI before and after an intravenous injection of USPIO-R. Subsequently, an aortic specimen from the mice was removed and sliced for fluorescence imaging and Prussian blue and immunofluorescent staining. Results: T2 signal loss appeared and persisted in the aortic plaque postinjection, and spontaneous fluorescence from the plaque was observed. The accumulated mechanism of USPIO-R by plaque was the macrophage internalization by Prussian blue and immunofluorescence. Conclusion: USPIO-R is a promising dual-modality probe for diagnosing and monitoring vulnerable atherosclerotic plaques | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a MRI | |
| 650 | 4 | |a atherosclerotic plaque | |
| 650 | 4 | |a fluorescence | |
| 650 | 4 | |a rhodamine | |
| 650 | 4 | |a ultrasmall superparamagnetic iron oxide | |
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| 650 | 7 | |a Dextrans |2 NLM | |
| 650 | 7 | |a Magnetite Nanoparticles |2 NLM | |
| 650 | 7 | |a Rhodamines |2 NLM | |
| 650 | 7 | |a ferumoxtran-10 |2 NLM | |
| 700 | 1 | |a Pan, Yutao |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Xiaodong |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Yuan |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Huoqiang |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Hong |e verfasserin |4 aut | |
| 700 | 1 | |a Jiang, Lei |e verfasserin |4 aut | |
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