Morphological and molecular differentiation by high resolution melting curves (HRMA) and sequencing of the Trichophyton mentagrophytes complex in human strains in Valparaíso, Chile
Copyright © 2019 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved..
Trichophyton mentagrophytes complex is the most frequent agent found in dermatophyte isolates after Trichophyton rubrum. It is divided into 3main groups: (1) Trichophyton benhamiae and related species; (2) Trichophyton simii and 2related species, Trichophyton quinckeanum and Trichophyton schoenleinii; and (3) T. mentagrophytes, T. interdigitale, and related species. They are all difficult to identify by morphophysiology. With the aim of classifying them at the species level, a descriptive study was performed on 17 isolated clinical strains identified in the Mycology Laboratory of the Universidad de Valparaíso as belonging to the T. mentagrophytes complex. They were compared with 3 reference strains (T. mentagrophytes CBS 318.56, T. interdigitale CBS 428.63, Trichophyton erinacei CBS 511.73). Morphophysiological and molecular tests were performed by high resolution melting analysis curves and ITS regions sequencing. Phenotypically, 3 species of the complex were identified. Molecular analyses reclassified all the species as belonging to T. interdigitale. In conclusion, no reliable morphophysiological patterns were established to differentiate between the species of the complex. Molecularly, all the strains studied were classified as T. interdigitale.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:52 |
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| Enthalten in: |
Revista Argentina de microbiologia - 52(2020), 2 vom: 15. Apr., Seite 88-95 |
| Sprache: |
Spanisch |
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| Weiterer Titel: |
Diferenciación morfofisiológica y molecular por curvas de melting de alta resolución (HRMA) y secuenciación del complejo Trichophyton mentagrophytes en cepas humanas en Valparaíso, Chile |
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| Beteiligte Personen: |
Vieille Oyarzo, Peggy [VerfasserIn] |
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| Links: |
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| Themen: |
Comparative Study |
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| Anmerkungen: |
Date Completed 07.07.2021 Date Revised 07.07.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.ram.2019.05.002 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM299617432 |
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| 245 | 1 | 0 | |a Morphological and molecular differentiation by high resolution melting curves (HRMA) and sequencing of the Trichophyton mentagrophytes complex in human strains in Valparaíso, Chile |
| 246 | 3 | 3 | |a Diferenciación morfofisiológica y molecular por curvas de melting de alta resolución (HRMA) y secuenciación del complejo Trichophyton mentagrophytes en cepas humanas en Valparaíso, Chile |
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| 500 | |a Date Revised 07.07.2021 | ||
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2019 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved. | ||
| 520 | |a Trichophyton mentagrophytes complex is the most frequent agent found in dermatophyte isolates after Trichophyton rubrum. It is divided into 3main groups: (1) Trichophyton benhamiae and related species; (2) Trichophyton simii and 2related species, Trichophyton quinckeanum and Trichophyton schoenleinii; and (3) T. mentagrophytes, T. interdigitale, and related species. They are all difficult to identify by morphophysiology. With the aim of classifying them at the species level, a descriptive study was performed on 17 isolated clinical strains identified in the Mycology Laboratory of the Universidad de Valparaíso as belonging to the T. mentagrophytes complex. They were compared with 3 reference strains (T. mentagrophytes CBS 318.56, T. interdigitale CBS 428.63, Trichophyton erinacei CBS 511.73). Morphophysiological and molecular tests were performed by high resolution melting analysis curves and ITS regions sequencing. Phenotypically, 3 species of the complex were identified. Molecular analyses reclassified all the species as belonging to T. interdigitale. In conclusion, no reliable morphophysiological patterns were established to differentiate between the species of the complex. Molecularly, all the strains studied were classified as T. interdigitale | ||
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| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a Secuenciación | |
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