Transgene stacking in potato using the GAANTRY system
OBJECTIVE: GAANTRY (Gene Assembly in Agrobacterium by Nucleic acid Transfer using Recombinase technologY) is a flexible and effective system for stably stacking multiple genes within an Agrobacterium virulence plasmid Transfer-DNA (T-DNA). We examined the ability of the GAANTRY Agrobacterium rhizogenes ArPORT1 '10-stack' strain to generate transgenic potato plants.
RESULTS: The 28.5 kilobase 10-stack T-DNA, was introduced into Lenape potato plants with a 32% transformation efficiency. Molecular and phenotypic characterization confirmed that six of the seven tested independent transgenic lines carried the entire desired construct, demonstrating that the GAANTRY 10-stack strain can be used can be used in a tissue culture-based callus transformation method to efficiently generate transgenic potato plants. Analysis using droplet digital PCR showed that most of the characterized events carry one or two copies of the 10-stack transgenes and that 'backbone' DNA from outside of the T-DNA was absent in the transgenic plants. These results demonstrate that the GAANTRY system efficiently generates high quality transgenic potato plants with a large construct of stacked transgenes.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:12 |
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| Enthalten in: |
BMC research notes - 12(2019), 1 vom: 25. Juli, Seite 457 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
McCue, Kent F [VerfasserIn] |
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| Links: |
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| Themen: |
DNA, Bacterial |
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| Anmerkungen: |
Date Completed 06.01.2020 Date Revised 13.12.2023 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1186/s13104-019-4493-8 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM299571335 |
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| 245 | 1 | 0 | |a Transgene stacking in potato using the GAANTRY system |
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| 500 | |a Date Revised 13.12.2023 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a OBJECTIVE: GAANTRY (Gene Assembly in Agrobacterium by Nucleic acid Transfer using Recombinase technologY) is a flexible and effective system for stably stacking multiple genes within an Agrobacterium virulence plasmid Transfer-DNA (T-DNA). We examined the ability of the GAANTRY Agrobacterium rhizogenes ArPORT1 '10-stack' strain to generate transgenic potato plants | ||
| 520 | |a RESULTS: The 28.5 kilobase 10-stack T-DNA, was introduced into Lenape potato plants with a 32% transformation efficiency. Molecular and phenotypic characterization confirmed that six of the seven tested independent transgenic lines carried the entire desired construct, demonstrating that the GAANTRY 10-stack strain can be used can be used in a tissue culture-based callus transformation method to efficiently generate transgenic potato plants. Analysis using droplet digital PCR showed that most of the characterized events carry one or two copies of the 10-stack transgenes and that 'backbone' DNA from outside of the T-DNA was absent in the transgenic plants. These results demonstrate that the GAANTRY system efficiently generates high quality transgenic potato plants with a large construct of stacked transgenes | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a Gene-stacking | |
| 650 | 4 | |a Site-specific recombinase | |
| 650 | 4 | |a Solanum tuberosum | |
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| 700 | 1 | |a Gardner, Ethan |e verfasserin |4 aut | |
| 700 | 1 | |a Chan, Ronald |e verfasserin |4 aut | |
| 700 | 1 | |a Thilmony, Roger |e verfasserin |4 aut | |
| 700 | 1 | |a Thomson, James |e verfasserin |4 aut | |
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