Improvement in bioactive protein storage stability and colon-targeted release : a simple double-layer chitosan-based particle
Aim: To improve the long-term storage stability of a bioactive protein and the subsequent colon-targeted release, a double-layer chitosan (CS)-based particle was developed. Methods: To form the double-layer CS-based particle, the second layer was crosslinked onto the single-layer bovine serum albumin (BSA)-loaded CS-based particle. The structure and properties of particles were further investigated. Results: With the second layer, the double-layer particles became more compact, which was important for the inhibition of bioactive protein leakage during storage through strong electrostatic interactions and swelling of the hydrogels. After 30 d of storage, there was only 43.74-49.32% BSA leakage from the C15-TPP/C15-HMP double-layer particles. Moreover, the BSA release in subsequent colon-targeted delivery after storage was 44.02-48.59%. Conclusions: With double-layer shielding and a more compact arrangement, it was possible to reduce bioactive protein leakage over long periods storage and achieve subsequent colon-targeted delivery.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:36 |
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| Enthalten in: |
Journal of microencapsulation - 36(2019), 5 vom: 02. Aug., Seite 474-484 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Xiang, Tuo [VerfasserIn] |
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| Links: |
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| Themen: |
27432CM55Q |
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| Anmerkungen: |
Date Completed 27.01.2020 Date Revised 27.01.2020 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1080/02652048.2019.1646336 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM299308022 |
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| 245 | 1 | 0 | |a Improvement in bioactive protein storage stability and colon-targeted release |b a simple double-layer chitosan-based particle |
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| 500 | |a Date Completed 27.01.2020 | ||
| 500 | |a Date Revised 27.01.2020 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Aim: To improve the long-term storage stability of a bioactive protein and the subsequent colon-targeted release, a double-layer chitosan (CS)-based particle was developed. Methods: To form the double-layer CS-based particle, the second layer was crosslinked onto the single-layer bovine serum albumin (BSA)-loaded CS-based particle. The structure and properties of particles were further investigated. Results: With the second layer, the double-layer particles became more compact, which was important for the inhibition of bioactive protein leakage during storage through strong electrostatic interactions and swelling of the hydrogels. After 30 d of storage, there was only 43.74-49.32% BSA leakage from the C15-TPP/C15-HMP double-layer particles. Moreover, the BSA release in subsequent colon-targeted delivery after storage was 44.02-48.59%. Conclusions: With double-layer shielding and a more compact arrangement, it was possible to reduce bioactive protein leakage over long periods storage and achieve subsequent colon-targeted delivery | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Double-layer particle | |
| 650 | 4 | |a bioactive protein | |
| 650 | 4 | |a colon-targeted release | |
| 650 | 4 | |a crosslinked chitosan | |
| 650 | 4 | |a storage stability | |
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| 650 | 7 | |a Serum Albumin, Bovine |2 NLM | |
| 650 | 7 | |a 27432CM55Q |2 NLM | |
| 650 | 7 | |a Chitosan |2 NLM | |
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| 700 | 1 | |a Yang, Jingwen |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Shanshan |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Jiaying |e verfasserin |4 aut | |
| 700 | 1 | |a Situ, Wenbei |e verfasserin |4 aut | |
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