The Activin-like ligand Dawdle regulates innate immune responses through modulating NF-κB signaling in mud crab Scylla paramamosain
Copyright © 2019 Elsevier Ltd. All rights reserved..
Activins, members of transforming growth factor β (TGF-β) superfamily, are pleiotropic cytokines with critical roles in mediating cell proliferation, differentiation, homeostasis, apoptosis and immune response. However, the structural characteristics and specific functions of Activins remain largely unknown in invertebrates. In the present study, an Activin-like ligand Dawdle (Daw) was firstly identified and characterized from mud crab Scylla paramamosain. The obtained cDNA sequence of SpDaw was 2, 196 bp long with a 1, 149 bp open reading fame, which encoded a putative protein of 382 amino acids. The putative SpDaw protein contained a signal peptide, a TGF-β propeptide region and a TGF-β domain. Real-time PCR analysis demonstrated that SpDaw was predominantly expressed at early embryonic development stage and premolt stages, implying its participation in development and growth. Furthermore, SpDaw responded to both Vibro alginolyticus and Poly (I:C) challenges, suggesting the involvement of SpDaw in innate immune responses. Knockdown of SpDaw in vivo dramatically increased the expressions of NF-κB signaling genes and anti-lipopolysaccharide factor (ALF) genes, and the bacteria clearance efficiency was also markedly enhanced in SpDaw-silenced crabs. Moreover, the in vitro experiment further demonstrated that recombinant SpDaw protein could block the increased transcription of IKKs, NF-κBs and ALFs induced by pathogen challenges. Taken together, these results indicated that SpDaw not only participated in development and growth processes but also played an immune-regulatory role in crabs' innate immunity, which may pave the way for a better understanding of TGF-β superfamily members in crustacean species.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:101 |
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| Enthalten in: |
Developmental and comparative immunology - 101(2019) vom: 28. Dez., Seite 103450 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhou, Yi-Lian [VerfasserIn] |
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| Links: |
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| Themen: |
104625-48-1 |
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| Anmerkungen: |
Date Completed 20.04.2020 Date Revised 20.04.2020 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.dci.2019.103450 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM299196569 |
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| 100 | 1 | |a Zhou, Yi-Lian |e verfasserin |4 aut | |
| 245 | 1 | 4 | |a The Activin-like ligand Dawdle regulates innate immune responses through modulating NF-κB signaling in mud crab Scylla paramamosain |
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| 520 | |a Copyright © 2019 Elsevier Ltd. All rights reserved. | ||
| 520 | |a Activins, members of transforming growth factor β (TGF-β) superfamily, are pleiotropic cytokines with critical roles in mediating cell proliferation, differentiation, homeostasis, apoptosis and immune response. However, the structural characteristics and specific functions of Activins remain largely unknown in invertebrates. In the present study, an Activin-like ligand Dawdle (Daw) was firstly identified and characterized from mud crab Scylla paramamosain. The obtained cDNA sequence of SpDaw was 2, 196 bp long with a 1, 149 bp open reading fame, which encoded a putative protein of 382 amino acids. The putative SpDaw protein contained a signal peptide, a TGF-β propeptide region and a TGF-β domain. Real-time PCR analysis demonstrated that SpDaw was predominantly expressed at early embryonic development stage and premolt stages, implying its participation in development and growth. Furthermore, SpDaw responded to both Vibro alginolyticus and Poly (I:C) challenges, suggesting the involvement of SpDaw in innate immune responses. Knockdown of SpDaw in vivo dramatically increased the expressions of NF-κB signaling genes and anti-lipopolysaccharide factor (ALF) genes, and the bacteria clearance efficiency was also markedly enhanced in SpDaw-silenced crabs. Moreover, the in vitro experiment further demonstrated that recombinant SpDaw protein could block the increased transcription of IKKs, NF-κBs and ALFs induced by pathogen challenges. Taken together, these results indicated that SpDaw not only participated in development and growth processes but also played an immune-regulatory role in crabs' innate immunity, which may pave the way for a better understanding of TGF-β superfamily members in crustacean species | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Activin | |
| 650 | 4 | |a Dawdle | |
| 650 | 4 | |a Immune response | |
| 650 | 4 | |a Scylla paramamosain | |
| 650 | 4 | |a TGF-β superfamily | |
| 650 | 7 | |a Arthropod Proteins |2 NLM | |
| 650 | 7 | |a Carrier Proteins |2 NLM | |
| 650 | 7 | |a Ligands |2 NLM | |
| 650 | 7 | |a NF-kappa B |2 NLM | |
| 650 | 7 | |a Activins |2 NLM | |
| 650 | 7 | |a 104625-48-1 |2 NLM | |
| 700 | 1 | |a Li, Bo |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Ya-Ping |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Lan-Zhi |e verfasserin |4 aut | |
| 700 | 1 | |a Gu, Wen-Bin |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Ze-Peng |e verfasserin |4 aut | |
| 700 | 1 | |a Dong, Wei-Ren |e verfasserin |4 aut | |
| 700 | 1 | |a Shu, Miao-An |e verfasserin |4 aut | |
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