Dual-responsive nanoparticles based on chitosan for enhanced breast cancer therapy
Copyright © 2019 Elsevier Ltd. All rights reserved..
In this study, we report a novel pH and temperature responsive paclitaxel-loaded drug delivery system based on chitosan and di(ethylene glycol) methyl ether methacrylate. This was functionalized with hyaluronic acid to permit active targeting of CD44-overexpressing human breast cancer cells. The resultant HA-CS-g-PDEGMA-PTX nanoparticles (NPs) have small and uniform sizes (˜170 nm), a high drug loading (13.6 ± 1.3%) and high encapsulation efficiency (76.2 ± 8.5%). Cell viability and confocal microscopy experiments demonstrated that the NPs could effectively target and kill MDA-MB-231 human breast cancer cells, but were much less toxic to healthy human umbilical vein endothelial cells. In vivo biodistribution studies in mice showed that the NPs accumulated in the tumor site, while free drug was distributed more widely and rapidly cleared from the body. Histopathological studies revealed that the NPs led to enhanced apoptosis in the tumor site, which resulted in reduced tumor growth. The NPs prepared in this work have great potential for the treatment of breast cancers, and further offer a platform with which to target other cancers.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:221 |
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| Enthalten in: |
Carbohydrate polymers - 221(2019) vom: 01. Okt., Seite 84-93 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhang, Xuejing [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 03.12.2019 Date Revised 03.12.2019 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.carbpol.2019.05.081 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Copyright © 2019 Elsevier Ltd. All rights reserved. | ||
| 520 | |a In this study, we report a novel pH and temperature responsive paclitaxel-loaded drug delivery system based on chitosan and di(ethylene glycol) methyl ether methacrylate. This was functionalized with hyaluronic acid to permit active targeting of CD44-overexpressing human breast cancer cells. The resultant HA-CS-g-PDEGMA-PTX nanoparticles (NPs) have small and uniform sizes (˜170 nm), a high drug loading (13.6 ± 1.3%) and high encapsulation efficiency (76.2 ± 8.5%). Cell viability and confocal microscopy experiments demonstrated that the NPs could effectively target and kill MDA-MB-231 human breast cancer cells, but were much less toxic to healthy human umbilical vein endothelial cells. In vivo biodistribution studies in mice showed that the NPs accumulated in the tumor site, while free drug was distributed more widely and rapidly cleared from the body. Histopathological studies revealed that the NPs led to enhanced apoptosis in the tumor site, which resulted in reduced tumor growth. The NPs prepared in this work have great potential for the treatment of breast cancers, and further offer a platform with which to target other cancers | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Anticancer efficacy | |
| 650 | 4 | |a Dual-responsive | |
| 650 | 4 | |a Hyaluronic acid | |
| 650 | 4 | |a Nanoparticles | |
| 650 | 7 | |a Antineoplastic Agents, Phytogenic |2 NLM | |
| 650 | 7 | |a Drug Carriers |2 NLM | |
| 650 | 7 | |a Ethylene Glycols |2 NLM | |
| 650 | 7 | |a Methacrylates |2 NLM | |
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| 700 | 1 | |a Wu, Jianrong |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Xia |e verfasserin |4 aut | |
| 700 | 1 | |a Zheng, Hong |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Li-Min |e verfasserin |4 aut | |
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