An iridium(III) complex/G-quadruplex ensemble for detection of ochratoxin A based on long-lifetime luminescent
Copyright © 2019. Published by Elsevier Inc..
A G-quadruplex-based platform has been developed for the time-resolved monitoring of ochratoxin A (OTA). The simple platform displays good sensitivity for OTA with a detection limit of 40 nM via steady-state emission spectroscopy. Notably, the platform showed a detection limit of 10.8 nM via time-resolved emission spectroscopy (TRES), which is about 4 times more sensitive than steady-state mode. Moreover, the probe showed excellent selectivity for OTA over other mycotoxins. Furthermore, OTA was successfully detected in actual herbal plant extracts samples. Our platform is the first to detect OTA using TRES to distinguish between the target signals versus the auto-fluorescence of real samples. This platform shows improved detection speed, accuracy and sensitivity with simple operation, low cost, and no requirement for complicated pre-processing.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:580 |
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| Enthalten in: |
Analytical biochemistry - 580(2019) vom: 01. Sept., Seite 49-55 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhang, Jia-Tong [VerfasserIn] |
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| Links: |
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| Themen: |
1779SX6LUY |
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| Anmerkungen: |
Date Completed 06.04.2020 Date Revised 08.04.2020 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.ab.2019.06.005 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM298116294 |
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| 100 | 1 | |a Zhang, Jia-Tong |e verfasserin |4 aut | |
| 245 | 1 | 3 | |a An iridium(III) complex/G-quadruplex ensemble for detection of ochratoxin A based on long-lifetime luminescent |
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| 500 | |a Date Revised 08.04.2020 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2019. Published by Elsevier Inc. | ||
| 520 | |a A G-quadruplex-based platform has been developed for the time-resolved monitoring of ochratoxin A (OTA). The simple platform displays good sensitivity for OTA with a detection limit of 40 nM via steady-state emission spectroscopy. Notably, the platform showed a detection limit of 10.8 nM via time-resolved emission spectroscopy (TRES), which is about 4 times more sensitive than steady-state mode. Moreover, the probe showed excellent selectivity for OTA over other mycotoxins. Furthermore, OTA was successfully detected in actual herbal plant extracts samples. Our platform is the first to detect OTA using TRES to distinguish between the target signals versus the auto-fluorescence of real samples. This platform shows improved detection speed, accuracy and sensitivity with simple operation, low cost, and no requirement for complicated pre-processing | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a G-quadruplex | |
| 650 | 4 | |a Herbal plant extracts | |
| 650 | 4 | |a OTA | |
| 650 | 4 | |a TRES | |
| 650 | 7 | |a Aptamers, Nucleotide |2 NLM | |
| 650 | 7 | |a Ochratoxins |2 NLM | |
| 650 | 7 | |a ochratoxin A |2 NLM | |
| 650 | 7 | |a 1779SX6LUY |2 NLM | |
| 650 | 7 | |a Iridium |2 NLM | |
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| 700 | 1 | |a Kang, Tian-Shu |e verfasserin |4 aut | |
| 700 | 1 | |a Wong, Suk-Yu |e verfasserin |4 aut | |
| 700 | 1 | |a Pei, Ren-Jun |e verfasserin |4 aut | |
| 700 | 1 | |a Ma, Dik-Lung |e verfasserin |4 aut | |
| 700 | 1 | |a Leung, Chung-Hang |e verfasserin |4 aut | |
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